biostudies-literatureEmanuel RVConsejo Nacional de Ciencia y Tecnología, México329https://www.ebi.ac.uk/biostudies/studies/S-EPMC7334336biostudies-literatureUnknown10(8)The co-culture of plant beneficial microbes to stimulate the production of antimicrobial metabolites is gaining ground. Here, the inactivated <i>Colletotrichum gloeosporioides</i> mycelium was used to induce the biosynthesis of antifungal compounds in the co-culture systems of <i>Trichoderma</i> sp. and <i>Bacillus subtilis</i>. The hexanic extracts obtained from the co-culture systems were tested against <i>C. gloeosporioides.</i> Those that inhibited the phytopathogen growth were further fractionated by column and thin-layer chromatography and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Ethyl butanoate, butyl acetate, acetic acid, 2-butoxyethanol, 3,5-di-tert-butyl-4-hydroxybenzaldehyde, 3,5-di-tert-butyl-4-hydroxybenzyl alcohol, hexadecanoic acid, and octadecano3 BiotechIn vitro growth of <i>Colletotrichum gloeosporioides</i> is affected by butyl acetate, a compound produced during the co-culture of <i>Trichoderma</i> sp. and <i>Bacillus subtilis</i>.PMC7334336222405Cesar Arturo PULourdes Iveth MRMauricio Nahuam CAEmanuel RVHomero RCfalseIn vitro growth of <i>Colletotrichum gloeosporioides</i> is affected by butyl acetate, a compound produced during the co-culture of <i>Trichoderma</i> sp. and <i>Bacillus subtilis</i>.The co-culture of plant beneficial microbes to stimulate the production of antimicrobial metabolites is gaining ground. Here, the inactivated <i>Colletotrichum gloeosporioides</i> mycelium was used to induce the biosynthesis of antifungal compounds in the co-culture systems of <i>Trichoderma</i> sp. and <i>Bacillus subtilis</i>. The hexanic extracts obtained from the co-culture systems were tested against <i>C. gloeosporioides.</i> Those that inhibited the phytopathogen growth were further fractionated by column and thin-layer chromatography and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Ethyl butanoate, butyl acetate, acetic acid, 2-butoxyethanol, 3,5-di-tert-butyl-4-hydroxybenzaldehyde, 3,5-di-tert-butyl-4-hydroxybenzyl alcohol, hexadecanoic acid, and octadecano2020-01-01T00:00:00Z2020 Aug2025-04-05T12:03:36.682Z2022-02-11T00:16:13.768ZS-EPMC73343363265606210.1007/s13205-020-02324-z