biostudies-literatureUnknown23(7)Papakyrikos AMNational Center for Research ResourcesStanford UniversityU.S. Department of DefenseNational Science FoundationThe fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal air filling. PTPMT1 is a widely conserved, ubiquitously expressed mitochondrial phosphatase. To reveal PTPMT1's functions in genetically tractable invertebrates and whether those functions are tissue specific, we generate a Drosophila model of PTPMT1 depletion. We find that fly PTPMT1 mutants show impairments in tracheal air filling and subsequent activation of innate immune responses. On a cellular level, these defects are preceded by aggregation of mitochondria within the tracheal epithelial cells. OuriScience101285https://www.ebi.ac.uk/biostudies/studies/S-EPMC7334580biostudies-literatureDrosophila PTPMT1 Has a Function in Tracheal Air Filling.PMC7334580Kim MJWang XPapakyrikos AMfalseDrosophila PTPMT1 Has a Function in Tracheal Air Filling.The fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal air filling. PTPMT1 is a widely conserved, ubiquitously expressed mitochondrial phosphatase. To reveal PTPMT1's functions in genetically tractable invertebrates and whether those functions are tissue specific, we generate a Drosophila model of PTPMT1 depletion. We find that fly PTPMT1 mutants show impairments in tracheal air filling and subsequent activation of innate immune responses. On a cellular level, these defects are preceded by aggregation of mitochondria within the tracheal epithelial cells. Our2020-01-01T00:00:00Z2020 Jul2026-05-07T16:31:27.873Z2020-07-11T07:05:33ZS-EPMC73345803262942110.1016/j.isci.2020.101285