biostudies-literatureNerozzi CMinisterio de Ciencia, Innovación y UniversidadesAgència de Gestió d&apos;Ajuts Universitaris i de RecercaUniversitat de Girona11026https://www.ebi.ac.uk/biostudies/studies/S-EPMC7335210biostudies-literatureUnknown10(1)The wide use of glyphosate-based herbicides (GBHs) has become a matter of concern due to its potential harmful effects on human health, including men fertility. This study sought to investigate, using the pig as a model, the impact of pure glyphosate and its most known commercial formulation, Roundup, on sperm function and survival. With this purpose, fresh commercial semen doses were incubated with different concentrations (0-360 µg/mL) of glyphosate (GLY; exp. 1) or Roundup, at the equivalent GLY concentration (exp. 2), at 38 °C for 3 h. Glyphosate at 360 µg/mL significantly (P < 0.05) decreased sperm motility, viability, mitochondrial activity and acrosome integrity but had no detrimental effect at lower doses. On the other hand, Roundup did significantly (P < 0.05) reduce sperm motilitScientific reportsEffects of Roundup and its main component, glyphosate, upon mammalian sperm function and survival.PMC73352102017-SGR-1229RYC-2014-15581IFUdG2018Recuero SNerozzi CBucci DYeste MGaleati GSpinaci MfalseEffects of Roundup and its main component, glyphosate, upon mammalian sperm function and survival.The wide use of glyphosate-based herbicides (GBHs) has become a matter of concern due to its potential harmful effects on human health, including men fertility. This study sought to investigate, using the pig as a model, the impact of pure glyphosate and its most known commercial formulation, Roundup, on sperm function and survival. With this purpose, fresh commercial semen doses were incubated with different concentrations (0-360 µg/mL) of glyphosate (GLY; exp. 1) or Roundup, at the equivalent GLY concentration (exp. 2), at 38 °C for 3 h. Glyphosate at 360 µg/mL significantly (P < 0.05) decreased sperm motility, viability, mitochondrial activity and acrosome integrity but had no detrimental effect at lower doses. On the other hand, Roundup did significantly (P < 0.05) reduce sperm motilit2020-01-01T00:00:00Z2020 Jul2025-04-04T14:25:14.283Z2020-07-11T07:05:53ZS-EPMC73352103262084810.1038/s41598-020-67538-w