{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Longobardi V"],"funding":["This research was funded by the project: Development of innovative pro-fertility products in bovine frozen semen, Nuproferb\". PSR Regione Umbria 2014-2020.","This research was funded by the project: Development of innovative pro-fertility products in bovine frozen semen, Nuproferb”. PSR Regione Umbria 2014-2020."],"pagination":["E1065"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7341314"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(6)"],"pubmed_abstract":["Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Forty-eight ejaculates, collected from eight bulls (six per bull), were analyzed by liquid chromatography-mass spectrometry. Cryopreservation resulted in an over-expression of lysophosphatidylcholine (0:0/18:2(9Z,12Z)) in seminal plasma. In addition, higher levels of glycine betaine and pyro-l-glutaminyl-l-glutamine were observed in cryopreserved compared to fresh spermatozoa. The fresh seminal plasma of high-fertility bulls showed an over-expression of l-acetylcarnitine, glycerol tripropanoate, 2,3"],"journal":["Animals : an open access journal from MDPI"],"pubmed_title":["Changes in Bull Semen Metabolome in Relation to Cryopreservation and Fertility."],"pmcid":["PMC7341314"],"funding_grant_id":["no grant number"],"pubmed_authors":["Vassetti A","Pagano N","Fatone G","Vinale F","Longobardi V","Staropoli A","Campanile G","Gasparrini B","Kosior MA"],"additional_accession":[]},"is_claimable":false,"name":"Changes in Bull Semen Metabolome in Relation to Cryopreservation and Fertility.","description":"Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Forty-eight ejaculates, collected from eight bulls (six per bull), were analyzed by liquid chromatography-mass spectrometry. Cryopreservation resulted in an over-expression of lysophosphatidylcholine (0:0/18:2(9Z,12Z)) in seminal plasma. In addition, higher levels of glycine betaine and pyro-l-glutaminyl-l-glutamine were observed in cryopreserved compared to fresh spermatozoa. The fresh seminal plasma of high-fertility bulls showed an over-expression of l-acetylcarnitine, glycerol tripropanoate, 2,3","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2026-07-15T23:34:32.849Z","creation":"2025-04-19T18:01:55.827Z"},"accession":"S-EPMC7341314","cross_references":{"pubmed":["32575657"],"doi":["10.3390/ani10061065"]}}