biostudies-literatureLongobardi VThis research was funded by the project: Development of innovative pro-fertility products in bovine frozen semen, Nuproferb". PSR Regione Umbria 2014-2020.This research was funded by the project: Development of innovative pro-fertility products in bovine frozen semen, Nuproferb”. PSR Regione Umbria 2014-2020.E1065https://www.ebi.ac.uk/biostudies/studies/S-EPMC7341314biostudies-literatureUnknown10(6)Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Forty-eight ejaculates, collected from eight bulls (six per bull), were analyzed by liquid chromatography-mass spectrometry. Cryopreservation resulted in an over-expression of lysophosphatidylcholine (0:0/18:2(9Z,12Z)) in seminal plasma. In addition, higher levels of glycine betaine and pyro-l-glutaminyl-l-glutamine were observed in cryopreserved compared to fresh spermatozoa. The fresh seminal plasma of high-fertility bulls showed an over-expression of l-acetylcarnitine, glycerol tripropanoate, 2,3Animals : an open access journal from MDPIChanges in Bull Semen Metabolome in Relation to Cryopreservation and Fertility.PMC7341314no grant numberVassetti APagano NFatone GVinale FLongobardi VStaropoli ACampanile GGasparrini BKosior MAfalseChanges in Bull Semen Metabolome in Relation to Cryopreservation and Fertility.Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Forty-eight ejaculates, collected from eight bulls (six per bull), were analyzed by liquid chromatography-mass spectrometry. Cryopreservation resulted in an over-expression of lysophosphatidylcholine (0:0/18:2(9Z,12Z)) in seminal plasma. In addition, higher levels of glycine betaine and pyro-l-glutaminyl-l-glutamine were observed in cryopreserved compared to fresh spermatozoa. The fresh seminal plasma of high-fertility bulls showed an over-expression of l-acetylcarnitine, glycerol tripropanoate, 2,32020-01-01T00:00:00Z2020 Jun2026-07-15T23:34:32.849Z2025-04-19T18:01:55.827ZS-EPMC73413143257565710.3390/ani10061065