{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Tan M"],"pubmed_abstract":["Gene knockdown techniques are widely used to examine the function of specific genes or proteins. While a variety of techniques are available, a technique commonly used on mammalian oocytes is mRNA knockdown by microinjection of small interfering RNA (siRNA), with non-specific siRNA injection used as a technical control. Here, we investigate whether and how the microinjection procedure itself affects the transcriptome of bovine oocytes. Injection of non-specific siRNA resulted in differential expression of 119 transcripts, of which 76 were down-regulated. Gene ontology analysis revealed that the differentially regulated genes were enriched in the biological processes of ATP synthesis, molecular transport and regulation of protein polyubiquitination. This study establishes a background effec"],"journal":["Scientific reports"],"pagination":["11211"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7343835"],"repository":["biostudies-literature"],"pubmed_title":["Microinjection induces changes in the transcriptome of bovine oocytes."],"pmcid":["PMC7343835"],"pubmed_authors":["Stout TAE","Tan M","Roelen BAJ","van Tol HTA","Mokry M"],"additional_accession":[]},"is_claimable":false,"name":"Microinjection induces changes in the transcriptome of bovine oocytes.","description":"Gene knockdown techniques are widely used to examine the function of specific genes or proteins. While a variety of techniques are available, a technique commonly used on mammalian oocytes is mRNA knockdown by microinjection of small interfering RNA (siRNA), with non-specific siRNA injection used as a technical control. Here, we investigate whether and how the microinjection procedure itself affects the transcriptome of bovine oocytes. Injection of non-specific siRNA resulted in differential expression of 119 transcripts, of which 76 were down-regulated. Gene ontology analysis revealed that the differentially regulated genes were enriched in the biological processes of ATP synthesis, molecular transport and regulation of protein polyubiquitination. This study establishes a background effec","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jul","modification":"2026-04-29T08:44:39.451Z","creation":"2025-04-07T09:04:20.766Z"},"accession":"S-EPMC7343835","cross_references":{"pubmed":["32641751"],"doi":["10.1038/s41598-020-67603-4"]}}