biostudies-literatureWei BChinese Government ScholarshipFonds Wetenschappelijk OnderzoekUniversiteit Gent777https://www.ebi.ac.uk/biostudies/studies/S-EPMC7343964biostudies-literatureUnknown11In proteins, hydrogen peroxide (H₂O₂) reacts with redox-sensitive cysteines to form cysteine sulfenic acid, also known as <i>S</i>-sulfenylation. These cysteine oxidation events can steer diverse cellular processes by altering protein interactions, trafficking, conformation, and function. Previously, we had identified <i>S</i>-sulfenylated proteins by using a tagged proteinaceous probe based on the yeast AP-1-like (Yap1) transcription factor that specifically reacts with sulfenic acids and traps them through a mixed disulfide bond. However, the identity of the <i>S</i>-sulfenylated amino acid residues within a protein remained enigmatic. By using the same transgenic YAP1C probe, we present here a technological advancement to identify <i>in situ</i> sulfenylated cysteiFrontiers in plant scienceIdentification of Sulfenylated Cysteines in <i>Arabidopsis thaliana</i> Proteins Using a Disulfide-Linked Peptide Reporter.PMC734396401J113112016066900361227020NG003809NG06916NG055416NYang JVan Breusegem FWei BMessens JWillems PHuang JTian CfalseIdentification of Sulfenylated Cysteines in <i>Arabidopsis thaliana</i> Proteins Using a Disulfide-Linked Peptide Reporter.In proteins, hydrogen peroxide (H₂O₂) reacts with redox-sensitive cysteines to form cysteine sulfenic acid, also known as <i>S</i>-sulfenylation. These cysteine oxidation events can steer diverse cellular processes by altering protein interactions, trafficking, conformation, and function. Previously, we had identified <i>S</i>-sulfenylated proteins by using a tagged proteinaceous probe based on the yeast AP-1-like (Yap1) transcription factor that specifically reacts with sulfenic acids and traps them through a mixed disulfide bond. However, the identity of the <i>S</i>-sulfenylated amino acid residues within a protein remained enigmatic. By using the same transgenic YAP1C probe, we present here a technological advancement to identify <i>in situ</i> sulfenylated cystei2020-01-01T00:00:00Z20202026-04-08T00:32:37.117Z2020-10-01T07:05:33ZS-EPMC73439643271434010.3389/fpls.2020.00777