biostudies-literatureDebunne NFonds Wetenschappelijk Onderzoek16120-16127https://www.ebi.ac.uk/biostudies/studies/S-EPMC7346264biostudies-literatureUnknown5(26)Finding adequate biomarkers for rapid and accurate disease detection, prognosis, and therapy is increasingly important. Quorum-sensing peptides are herein a new emerging group, produced by bacteria, fungi, protozoa, and viruses, with blood being the most straightforward sample type to detect/quantitate them. However, detailed information about suitable blood sample collection methods and storage conditions for measuring these quorum-sensing peptides hampers further clinical research and development. Here, we first tested the time-dependent stability of a set of chemically diverse quorum-sensing peptides, spiked in blood at different temperatures (4, 21, and 37 °C) in four different ethylenediamine tetraacetic acid (EDTA)-containing plasma tubes (with different protein-stabilizing additivesACS omegaInfluence of Blood Collection Methods and Long-Term Plasma Storage on Quorum-Sensing Peptide Stability.PMC73462641S21017N1158818NDebunne NDe Spiegeleer ADe Spiegeleer BDepuydt DJanssens YDescamps AWynendaele EfalseInfluence of Blood Collection Methods and Long-Term Plasma Storage on Quorum-Sensing Peptide Stability.Finding adequate biomarkers for rapid and accurate disease detection, prognosis, and therapy is increasingly important. Quorum-sensing peptides are herein a new emerging group, produced by bacteria, fungi, protozoa, and viruses, with blood being the most straightforward sample type to detect/quantitate them. However, detailed information about suitable blood sample collection methods and storage conditions for measuring these quorum-sensing peptides hampers further clinical research and development. Here, we first tested the time-dependent stability of a set of chemically diverse quorum-sensing peptides, spiked in blood at different temperatures (4, 21, and 37 °C) in four different ethylenediamine tetraacetic acid (EDTA)-containing plasma tubes (with different protein-stabilizing additives2020-01-01T00:00:00Z2020 Jul2025-04-22T19:36:32.961Z2025-04-06T02:47:03.555ZS-EPMC73462643265643410.1021/acsomega.0c01723