biostudies-literatureBlank HMNICHD NIH HHSNIDDK NIH HHSNIH HHSArmy Research LaboratoryNIGMS NIH HHS1069-1084https://www.ebi.ac.uk/biostudies/studies/S-EPMC7346729biostudies-literatureUnknown31(10)Establishing the pattern of abundance of molecules of interest during cell division has been a long-standing goal of cell cycle studies. Here, for the first time in any system, we present experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from unarrested, growing, and synchronously dividing yeast cells. Overall, transcript and protein levels were correlated, but specific processes that appeared to change at the RNA level (e.g., ribosome biogenesis) did not do so at the protein level, and vice versa. We also found no significant changes in codon usage or the ribosome content during the cell cycle. We describe an unexpected mitotic peak in the abundance of ergosterol and thiamine biosynthesis enzymes. Although the levels of several metabolites changed in theMolecular biology of the cellAbundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism.PMC7346729S10 OD016281R01 GM123139R01 DK110520U24 DK097154W911NF-17-2-0091S10 OD028654R35 GM122480R01 HD085901Blank HMPapoulas OMaitra NPolymenis MKennedy BKGarge RSchilling BMarcotte EMfalseAbundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism.Establishing the pattern of abundance of molecules of interest during cell division has been a long-standing goal of cell cycle studies. Here, for the first time in any system, we present experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from unarrested, growing, and synchronously dividing yeast cells. Overall, transcript and protein levels were correlated, but specific processes that appeared to change at the RNA level (e.g., ribosome biogenesis) did not do so at the protein level, and vice versa. We also found no significant changes in codon usage or the ribosome content during the cell cycle. We describe an unexpected mitotic peak in the abundance of ergosterol and thiamine biosynthesis enzymes. Although the levels of several metabolites changed in the2020-01-01T00:00:00Z2020 May2026-04-12T19:47:41.831Z2025-02-19T00:48:38.538ZS-EPMC73467293212970610.1091/mbc.E19-12-0708