biostudies-literatureUnknown11(6)Zhang YDrought stress is a major environmental stress, which adversely affects the biological and molecular processes of plants, thereby impairing their growth and development. In the present study, we found that the expression level of <i>OsTBP2.2</i> which encodes for a nucleus-localized protein member belonging to transcription factor IID (TFIID) family, was significantly induced by polyethylene glycol (PEG) treatment. Therefore, knockdown mutants of <i>OsTBP2.2</i> gene were generated to investigate the role of OsTBP2.2 in rice response to drought stress. Under the condition of drought stress, the photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance were significantly reduced in <i>ostbp2.2</i> lines compared with wild type, Dongjin (WT-DJ). Furthermore, theGenesE629https://www.ebi.ac.uk/biostudies/studies/S-EPMC7349065biostudies-literatureKnockdown of a Novel Gene <i>OsTBP2.2</i> Increases Sensitivity to Drought Stress in Rice.PMC7349065Zhao LFan XZhang YXiao HXiang JChew JQian KfalseKnockdown of a Novel Gene <i>OsTBP2.2</i> Increases Sensitivity to Drought Stress in Rice.Drought stress is a major environmental stress, which adversely affects the biological and molecular processes of plants, thereby impairing their growth and development. In the present study, we found that the expression level of <i>OsTBP2.2</i> which encodes for a nucleus-localized protein member belonging to transcription factor IID (TFIID) family, was significantly induced by polyethylene glycol (PEG) treatment. Therefore, knockdown mutants of <i>OsTBP2.2</i> gene were generated to investigate the role of OsTBP2.2 in rice response to drought stress. Under the condition of drought stress, the photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance were significantly reduced in <i>ostbp2.2</i> lines compared with wild type, Dongjin (WT-DJ). Furthermore, the2020-01-01T00:00:00Z2020 Jun2025-04-19T21:08:53.124Z2025-02-19T00:50:51.668ZS-EPMC73490653252171710.3390/genes11060629