{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(3)"],"submitter":["Nattinen J"],"pubmed_abstract":["<h4>Purpose</h4>The purpose of this study was to examine the protein profile differences between capillary and Schirmer strip tear fluid samples.<h4>Methods</h4>Both capillary and Schirmer strip tear samples were collected from 31 healthy participants at the same visit, and the samples were analyzed with nanoflow liquid chromatography coupled with time-of-flight mass spectrometer (NanoLC-MSTOF), implementing a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). Sample type-specific and combined spectral libraries were used to evaluate the differences between the sample types in protein expression levels and biological functions.<h4>Results</h4>In proportion, more extracellular proteins connected to immune response were quantified from the cap"],"journal":["Translational vision science & technology"],"pagination":["16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7351636"],"repository":["biostudies-literature"],"pubmed_title":["Comparison of Capillary and Schirmer Strip Tear Fluid Sampling Methods Using SWATH-MS Proteomics Approach."],"pmcid":["PMC7351636"],"pubmed_authors":["Uusitalo H","Jylha A","Nattinen J","Aapola U","Vaajanen A"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of Capillary and Schirmer Strip Tear Fluid Sampling Methods Using SWATH-MS Proteomics Approach.","description":"<h4>Purpose</h4>The purpose of this study was to examine the protein profile differences between capillary and Schirmer strip tear fluid samples.<h4>Methods</h4>Both capillary and Schirmer strip tear samples were collected from 31 healthy participants at the same visit, and the samples were analyzed with nanoflow liquid chromatography coupled with time-of-flight mass spectrometer (NanoLC-MSTOF), implementing a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). Sample type-specific and combined spectral libraries were used to evaluate the differences between the sample types in protein expression levels and biological functions.<h4>Results</h4>In proportion, more extracellular proteins connected to immune response were quantified from the cap","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Feb","modification":"2025-04-19T21:09:53.228Z","creation":"2025-02-19T00:51:45.425Z"},"accession":"S-EPMC7351636","cross_references":{"pubmed":["32714642"],"doi":["10.1167/tvst.9.3.16"]}}