biostudies-literatureUnknown9(3)Nattinen J<h4>Purpose</h4>The purpose of this study was to examine the protein profile differences between capillary and Schirmer strip tear fluid samples.<h4>Methods</h4>Both capillary and Schirmer strip tear samples were collected from 31 healthy participants at the same visit, and the samples were analyzed with nanoflow liquid chromatography coupled with time-of-flight mass spectrometer (NanoLC-MSTOF), implementing a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). Sample type-specific and combined spectral libraries were used to evaluate the differences between the sample types in protein expression levels and biological functions.<h4>Results</h4>In proportion, more extracellular proteins connected to immune response were quantified from the capTranslational vision science & technology16https://www.ebi.ac.uk/biostudies/studies/S-EPMC7351636biostudies-literatureComparison of Capillary and Schirmer Strip Tear Fluid Sampling Methods Using SWATH-MS Proteomics Approach.PMC7351636Uusitalo HJylha ANattinen JAapola UVaajanen AfalseComparison of Capillary and Schirmer Strip Tear Fluid Sampling Methods Using SWATH-MS Proteomics Approach.<h4>Purpose</h4>The purpose of this study was to examine the protein profile differences between capillary and Schirmer strip tear fluid samples.<h4>Methods</h4>Both capillary and Schirmer strip tear samples were collected from 31 healthy participants at the same visit, and the samples were analyzed with nanoflow liquid chromatography coupled with time-of-flight mass spectrometer (NanoLC-MSTOF), implementing a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). Sample type-specific and combined spectral libraries were used to evaluate the differences between the sample types in protein expression levels and biological functions.<h4>Results</h4>In proportion, more extracellular proteins connected to immune response were quantified from the cap2020-01-01T00:00:00Z2020 Feb2025-04-19T21:09:53.228Z2025-02-19T00:51:45.425ZS-EPMC73516363271464210.1167/tvst.9.3.16