biostudies-literatureMorrison EMassachusetts Department of Fish and Game368https://www.ebi.ac.uk/biostudies/studies/S-EPMC7351954biostudies-literatureUnknown3(1)Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. The reversible nature of this modification makes it a prime candidate as a mechanism for regulating signal transduction in T-cell receptor signaling. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras signal transduction. Among these stimulation-dependent palmitoylation targets are the v-SNARE VAMP7, important for docking of vesicular LAT during TCR signaling, and the largely undescribed palmitoyl acyltransferase DHHC18 that is expressed in two isoforms in T cells. Using our newly developed On-Plate Palmitoylation Assay (OPPA), we show DHHC18 is capable of palmitoylating VAMP7 at Cys183. CeCommunications biologyDynamic palmitoylation events following T-cell receptor signaling.PMC7351954TRR186Kliche SHivroz CFreund CMorrison EBrugger BAlvaro-Benito MKrause EWegner TZheng AZucchetti AEfalseDynamic palmitoylation events following T-cell receptor signaling.Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. The reversible nature of this modification makes it a prime candidate as a mechanism for regulating signal transduction in T-cell receptor signaling. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras signal transduction. Among these stimulation-dependent palmitoylation targets are the v-SNARE VAMP7, important for docking of vesicular LAT during TCR signaling, and the largely undescribed palmitoyl acyltransferase DHHC18 that is expressed in two isoforms in T cells. Using our newly developed On-Plate Palmitoylation Assay (OPPA), we show DHHC18 is capable of palmitoylating VAMP7 at Cys183. Ce2020-01-01T00:00:00Z2020 Jul2026-07-15T23:41:23.307Z2025-04-05T12:43:45.011ZS-EPMC73519543265144010.1038/s42003-020-1063-5