{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Goodge K"],"funding":["National Institutes of Health","National Science Foundation"],"pagination":["E1172"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7353318"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(6)"],"pubmed_abstract":["As potential high surface area for selective capture in diagnostic or filtration devices, biotin-cellulose nanofiber membranes were fabricated to demonstrate the potential for specific and bio-orthogonal attachment of biomolecules onto nanofiber surfaces. Cellulose acetate was electrospun and substituted with alkyne groups in either a one- or two-step process. The alkyne reaction, confirmed by FTIR and Raman spectroscopy, was dependent on solvent ratio, time, and temperature. The two-step process maximized alkyne substitution in 10/90 volume per volume ratio (v/v) water to isopropanol at 50 °C after 6 h compared to the one-step process in 80/20 (v/v) at 50 °C after 48 h. Azide-biotin conjugate \"clicked\" with the alkyne-cellulose via copper-catalyzed alkyne-azide cycloaddition (CuAAC). The "],"journal":["Nanomaterials (Basel, Switzerland)"],"pubmed_title":["Biotin-Conjugated Cellulose Nanofibers Prepared via Copper-Catalyzed Alkyne-Azide Cycloaddition (CuAAC) \"Click\" Chemistry."],"pmcid":["PMC7353318"],"funding_grant_id":["DMR-1719875","1S10RR025502"],"pubmed_authors":["Goodge K","Frey M"],"additional_accession":[]},"is_claimable":false,"name":"Biotin-Conjugated Cellulose Nanofibers Prepared via Copper-Catalyzed Alkyne-Azide Cycloaddition (CuAAC) \"Click\" Chemistry.","description":"As potential high surface area for selective capture in diagnostic or filtration devices, biotin-cellulose nanofiber membranes were fabricated to demonstrate the potential for specific and bio-orthogonal attachment of biomolecules onto nanofiber surfaces. Cellulose acetate was electrospun and substituted with alkyne groups in either a one- or two-step process. The alkyne reaction, confirmed by FTIR and Raman spectroscopy, was dependent on solvent ratio, time, and temperature. The two-step process maximized alkyne substitution in 10/90 volume per volume ratio (v/v) water to isopropanol at 50 °C after 6 h compared to the one-step process in 80/20 (v/v) at 50 °C after 48 h. Azide-biotin conjugate \"clicked\" with the alkyne-cellulose via copper-catalyzed alkyne-azide cycloaddition (CuAAC). The ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2025-04-19T11:37:44.915Z","creation":"2025-04-19T11:37:44.915Z"},"accession":"S-EPMC7353318","cross_references":{"pubmed":["32560117"],"doi":["10.3390/nano10061172"]}}