biostudies-literatureGoodge KNational Institutes of HealthNational Science FoundationE1172https://www.ebi.ac.uk/biostudies/studies/S-EPMC7353318biostudies-literatureUnknown10(6)As potential high surface area for selective capture in diagnostic or filtration devices, biotin-cellulose nanofiber membranes were fabricated to demonstrate the potential for specific and bio-orthogonal attachment of biomolecules onto nanofiber surfaces. Cellulose acetate was electrospun and substituted with alkyne groups in either a one- or two-step process. The alkyne reaction, confirmed by FTIR and Raman spectroscopy, was dependent on solvent ratio, time, and temperature. The two-step process maximized alkyne substitution in 10/90 volume per volume ratio (v/v) water to isopropanol at 50 °C after 6 h compared to the one-step process in 80/20 (v/v) at 50 °C after 48 h. Azide-biotin conjugate "clicked" with the alkyne-cellulose via copper-catalyzed alkyne-azide cycloaddition (CuAAC). The Nanomaterials (Basel, Switzerland)Biotin-Conjugated Cellulose Nanofibers Prepared via Copper-Catalyzed Alkyne-Azide Cycloaddition (CuAAC) "Click" Chemistry.PMC7353318DMR-17198751S10RR025502Goodge KFrey MfalseBiotin-Conjugated Cellulose Nanofibers Prepared via Copper-Catalyzed Alkyne-Azide Cycloaddition (CuAAC) "Click" Chemistry.As potential high surface area for selective capture in diagnostic or filtration devices, biotin-cellulose nanofiber membranes were fabricated to demonstrate the potential for specific and bio-orthogonal attachment of biomolecules onto nanofiber surfaces. Cellulose acetate was electrospun and substituted with alkyne groups in either a one- or two-step process. The alkyne reaction, confirmed by FTIR and Raman spectroscopy, was dependent on solvent ratio, time, and temperature. The two-step process maximized alkyne substitution in 10/90 volume per volume ratio (v/v) water to isopropanol at 50 °C after 6 h compared to the one-step process in 80/20 (v/v) at 50 °C after 48 h. Azide-biotin conjugate "clicked" with the alkyne-cellulose via copper-catalyzed alkyne-azide cycloaddition (CuAAC). The 2020-01-01T00:00:00Z2020 Jun2025-04-19T11:37:44.915Z2025-04-19T11:37:44.915ZS-EPMC73533183256011710.3390/nano10061172