{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Falchi FA"],"funding":["Università degli Studi di Napoli Federico II","Università degli Studì di Milano"],"pagination":["E826"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7356881"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(6)"],"pubmed_abstract":["LpxT is an inner membrane protein that transfers a phosphate group from the essential lipid undecaprenyl pyrophosphate (C-55PP) to the lipid A moiety of lipopolysaccharide, generating a lipid A <i>tris</i>-phosphorylated species. The protein is encoded by the non-essential <i>lpxT</i> gene, which is conserved in distantly related Gram-negative bacteria. In this work, we investigated the phenotypic effect of <i>lpxT</i> ectopic expression from a plasmid in <i>Escherichia coli</i>. We found that <i>lpxT</i> induction inhibited cell division and led to the formation of elongated cells, mostly with absent or altered septa. Moreover, the cells became sensitive to detergents and to hypo-osmotic shock, indicating that they had cell envelope defects. These effects were not due to lipid A hyperphos"],"journal":["Microorganisms"],"pubmed_title":["Overexpression of <i>lpxT</i> Gene in <i>Escherichia coli</i> Inhibits Cell Division and Causes Envelope Defects without Changing the Overall Phosphorylation Level of Lipid A."],"pmcid":["PMC7356881"],"funding_grant_id":["E66C18001330003","na"],"pubmed_authors":["Molinaro A","Briani F","Paroni M","Falchi FA","Di Lorenzo F","Pizzoccheri R","Forti F","Casino G"],"additional_accession":[]},"is_claimable":false,"name":"Overexpression of <i>lpxT</i> Gene in <i>Escherichia coli</i> Inhibits Cell Division and Causes Envelope Defects without Changing the Overall Phosphorylation Level of Lipid A.","description":"LpxT is an inner membrane protein that transfers a phosphate group from the essential lipid undecaprenyl pyrophosphate (C-55PP) to the lipid A moiety of lipopolysaccharide, generating a lipid A <i>tris</i>-phosphorylated species. The protein is encoded by the non-essential <i>lpxT</i> gene, which is conserved in distantly related Gram-negative bacteria. In this work, we investigated the phenotypic effect of <i>lpxT</i> ectopic expression from a plasmid in <i>Escherichia coli</i>. We found that <i>lpxT</i> induction inhibited cell division and led to the formation of elongated cells, mostly with absent or altered septa. Moreover, the cells became sensitive to detergents and to hypo-osmotic shock, indicating that they had cell envelope defects. These effects were not due to lipid A hyperphos","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 May","modification":"2026-04-29T08:43:46.4Z","creation":"2026-04-14T03:06:45.763Z"},"accession":"S-EPMC7356881","cross_references":{"pubmed":["32486329"],"doi":["10.3390/microorganisms8060826"]}}