biostudies-literatureKim YNational Research Foundation of KoreaE1694https://www.ebi.ac.uk/biostudies/studies/S-EPMC7356912biostudies-literatureUnknown9(6)This study aimed to understand the clinicopathological significance of runt-related transcription factor 1 (RUNX1) in non-small cell lung cancer (NSCLC). The methylation and mRNA levels of RUNX1 in NSCLC were determined using the Infinium HumanMethylation450 BeadChip and the HumanHT-12 expression BeadChip. RUNX1 protein levels were analyzed using immunohistochemistry of formalin-fixed paraffin-embedded tissues from 409 NSCLC patients. Three CpGs (cg04228935, cg11498607, and cg05000748) in the CpG island of RUNX1 showed significantly different methylation levels (Bonferroni corrected p < 0.05) between tumor and matched normal tissues obtained from 42 NSCLC patients. Methylation levels of the CpGs in the tumor tissues were inversely related to mRNA levels of RUNX1. A logistic regression modeJournal of clinical medicineClinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer.PMC73569122019R1F1A1057654Kim DHKim DUm SLee BBKim YCho EYHan JShim YMfalseClinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer.This study aimed to understand the clinicopathological significance of runt-related transcription factor 1 (RUNX1) in non-small cell lung cancer (NSCLC). The methylation and mRNA levels of RUNX1 in NSCLC were determined using the Infinium HumanMethylation450 BeadChip and the HumanHT-12 expression BeadChip. RUNX1 protein levels were analyzed using immunohistochemistry of formalin-fixed paraffin-embedded tissues from 409 NSCLC patients. Three CpGs (cg04228935, cg11498607, and cg05000748) in the CpG island of RUNX1 showed significantly different methylation levels (Bonferroni corrected p < 0.05) between tumor and matched normal tissues obtained from 42 NSCLC patients. Methylation levels of the CpGs in the tumor tissues were inversely related to mRNA levels of RUNX1. A logistic regression mode2020-01-01T00:00:00Z2020 Jun2025-04-26T08:15:30.594Z2025-04-06T12:37:05.165ZS-EPMC73569123249828810.3390/jcm9061694