biostudies-literatureCienfuegos-Pecina ELiver Unit and the Department of Analytical ChemistryNational Council of Science and Technologye9438https://www.ebi.ac.uk/biostudies/studies/S-EPMC7357568biostudies-literatureUnknown8<h4>Background</h4>Ischemia-reperfusion (IR) injury is the main cause of delayed graft function in solid organ transplantation. Hypoxia-inducible factors (HIFs) control the expression of genes related to preconditioning against IR injury. During normoxia, HIF-α subunits are marked for degradation by the egg-laying defective nine homolog (EGLN) family of prolyl-4-hydroxylases. The inhibition of EGLN stabilizes HIFs and protects against IR injury. The aim of this study was to determine whether the EGLN inhibitors sodium (<i>S</i>)-2-hydroxyglutarate [(<i>S</i>)-2HG] and succinic acid (SA) have a nephroprotective effect against renal IR injury in Wistar rats.<h4>Methods</h4>(<i>S</i>)-2HG was synthesized in a 22.96% yield from commercially available L-glutamic acid in a two-step methodology (PeerJEffect of sodium (<i>S</i>)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway.PMC73575682017-01-5652Munoz-Espinosa LEPerez-Rodriguez EDominguez-Vazquez IAlarcon-Galvan GSaucedo ALEsquivel-Figueroa DIbarra-Rivera TRRodriguez-Rodriguez DRAlcantara-Solano KJTorres-Gonzalez LRamirez-Martinez LACienfuegos-Pecina EMoreno-Pena DPCordero-Perez PfalseEffect of sodium (<i>S</i>)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway.<h4>Background</h4>Ischemia-reperfusion (IR) injury is the main cause of delayed graft function in solid organ transplantation. Hypoxia-inducible factors (HIFs) control the expression of genes related to preconditioning against IR injury. During normoxia, HIF-α subunits are marked for degradation by the egg-laying defective nine homolog (EGLN) family of prolyl-4-hydroxylases. The inhibition of EGLN stabilizes HIFs and protects against IR injury. The aim of this study was to determine whether the EGLN inhibitors sodium (<i>S</i>)-2-hydroxyglutarate [(<i>S</i>)-2HG] and succinic acid (SA) have a nephroprotective effect against renal IR injury in Wistar rats.<h4>Methods</h4>(<i>S</i>)-2HG was synthesized in a 22.96% yield from commercially available L-glutamic acid in a two-step methodology (2020-01-01T00:00:00Z20202025-04-19T18:01:37.166Z2025-04-19T18:01:37.166ZS-EPMC73575683272849110.7717/peerj.9438