biostudies-literatureArvin NENIDDK NIH HHSNational Institutes of HealthNIGMS NIH HHS1606-1616https://www.ebi.ac.uk/biostudies/studies/S-EPMC7357712biostudies-literatureUnknown12(12)Western blotting is a widely used protein assay platform, but the technique requires long analysis times and multiple manual steps. Microfluidic systems are currently being explored for increased automation and reduction of analysis times, sample volumes, and reagent consumption for western blots. Previous work has demonstrated that proteins separated by microchip electrophoresis can be captured on membranes by dragging the microchip outlet across the membrane. This process reduces the separation and transfer time of a western blot to a few minutes. To further improve the speed and miniaturization of a complete western blot, a microscale immunoassay with direct deposition of immunoassay reagents has been developed. Flow deposition of antibodies is used to overcome diffusion limited bindingAnalytical methods : advancing methods and applicationsFast Immunoassay for Microfluidic Western Blotting by Direct Deposition of Reagents onto Capture Membrane.PMC7357712R01 DK046960R43 GM1122891R43GM112289-01R44 GM112289R37 DK046960Dawod MArvin NEFurtaw MDKennedy RTLamb DTAnderson JPfalseFast Immunoassay for Microfluidic Western Blotting by Direct Deposition of Reagents onto Capture Membrane.Western blotting is a widely used protein assay platform, but the technique requires long analysis times and multiple manual steps. Microfluidic systems are currently being explored for increased automation and reduction of analysis times, sample volumes, and reagent consumption for western blots. Previous work has demonstrated that proteins separated by microchip electrophoresis can be captured on membranes by dragging the microchip outlet across the membrane. This process reduces the separation and transfer time of a western blot to a few minutes. To further improve the speed and miniaturization of a complete western blot, a microscale immunoassay with direct deposition of immunoassay reagents has been developed. Flow deposition of antibodies is used to overcome diffusion limited binding2020-01-01T00:00:00Z2020 Mar2025-04-04T01:42:46.991Z2025-04-04T01:42:46.991ZS-EPMC73577123266146410.1039/d0ay00207k