biostudies-literatureZhang JNatural Science Foundation of Beijing MunicipalityMinisterstvo Školství, Mládeže a TělovýchovyChina Postdoctoral Science FoundationBeijing Outstanding University Discipline Program3508https://www.ebi.ac.uk/biostudies/studies/S-EPMC7360611biostudies-literatureUnknown11(1)Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor- and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxiNature communicationsStrigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization.PMC7360611CEITEC 2020 (LQ1601)51920112019M660864Kalousek PReinohl VZhang JBalla JProchazka SHalouzka RFriml JLi YVasileva MTarkowski PGallei MBrewer PBMazur ELuschnig CWang YMedvedova ZPrat TfalseStrigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization.Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor- and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxi2020-01-01T00:00:00Z2020 Jul2025-04-19T21:07:20.089Z2025-02-19T00:50:40.491ZS-EPMC73606113266555410.1038/s41467-020-17252-y