{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(7)"],"submitter":["Chen W"],"pubmed_abstract":["Cell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock protein 90 (Hsp90), modulates cell migration by stabilizing both myosin-9 and lissencephaly protein 1 (LIS1). Either knockdown or knockout of NudCL2 significantly increases single-cell migration, but has no significant effect on collective cell migration. Immunoprecipitation-mass spectrometry and western blotting analyses reveal that NudCL2 binds to myosin-9 in mammalian cells. Depletion of NudCL2 not only decreases myosin-9 protein levels, but also results in actin disorganization. Ectopic expression of myosin-9 efficiently reverses defects in actin disorganization and single-cell migration in cells"],"journal":["Cell death & disease"],"pagination":["534"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7360774"],"repository":["biostudies-literature"],"pubmed_title":["NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90."],"pmcid":["PMC7360774"],"pubmed_authors":["Liu M","Zhou T","Sun X","Yang C","Xie S","Li M","Liu W","Wang L","Zhang W","Yang Y","Chen W","Wang W","Xu X"],"additional_accession":[]},"is_claimable":false,"name":"NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.","description":"Cell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock protein 90 (Hsp90), modulates cell migration by stabilizing both myosin-9 and lissencephaly protein 1 (LIS1). Either knockdown or knockout of NudCL2 significantly increases single-cell migration, but has no significant effect on collective cell migration. Immunoprecipitation-mass spectrometry and western blotting analyses reveal that NudCL2 binds to myosin-9 in mammalian cells. Depletion of NudCL2 not only decreases myosin-9 protein levels, but also results in actin disorganization. Ectopic expression of myosin-9 efficiently reverses defects in actin disorganization and single-cell migration in cells","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jul","modification":"2025-04-19T21:07:26.782Z","creation":"2025-02-19T00:50:54.362Z"},"accession":"S-EPMC7360774","cross_references":{"pubmed":["32665550"],"doi":["10.1038/s41419-020-02739-9"]}}