biostudies-literatureUnknown11(7)Chen WCell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock protein 90 (Hsp90), modulates cell migration by stabilizing both myosin-9 and lissencephaly protein 1 (LIS1). Either knockdown or knockout of NudCL2 significantly increases single-cell migration, but has no significant effect on collective cell migration. Immunoprecipitation-mass spectrometry and western blotting analyses reveal that NudCL2 binds to myosin-9 in mammalian cells. Depletion of NudCL2 not only decreases myosin-9 protein levels, but also results in actin disorganization. Ectopic expression of myosin-9 efficiently reverses defects in actin disorganization and single-cell migration in cellsCell death & disease534https://www.ebi.ac.uk/biostudies/studies/S-EPMC7360774biostudies-literatureNudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.PMC7360774Liu MZhou TSun XYang CXie SLi MLiu WWang LZhang WYang YChen WWang WXu XfalseNudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.Cell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock protein 90 (Hsp90), modulates cell migration by stabilizing both myosin-9 and lissencephaly protein 1 (LIS1). Either knockdown or knockout of NudCL2 significantly increases single-cell migration, but has no significant effect on collective cell migration. Immunoprecipitation-mass spectrometry and western blotting analyses reveal that NudCL2 binds to myosin-9 in mammalian cells. Depletion of NudCL2 not only decreases myosin-9 protein levels, but also results in actin disorganization. Ectopic expression of myosin-9 efficiently reverses defects in actin disorganization and single-cell migration in cells2020-01-01T00:00:00Z2020 Jul2025-04-19T21:07:26.782Z2025-02-19T00:50:54.362ZS-EPMC73607743266555010.1038/s41419-020-02739-9