biostudies-literatureUnknown12(6)Gevrek TNMultifunctionalizable hydrogel coatings on titanium interfaces are useful in a wide range of biomedical applications utilizing titanium-based materials. In this study, furan-protected maleimide groups containing multi-clickable biocompatible hydrogel layers are fabricated on a titanium surface. Upon thermal treatment, the masked maleimide groups within the hydrogel are converted to thiol-reactive maleimide groups. The thiol-reactive maleimide group allows facile functionalization of these hydrogels through the thiol-maleimide nucleophilic addition and Diels-Alder cycloaddition reactions, under mild conditions. Additionally, the strained alkene unit in the furan-protected maleimide moiety undergoes radical thiol-ene reaction, as well as the inverse-electron-demand Diels-Alder reaction with PolymersE1211https://www.ebi.ac.uk/biostudies/studies/S-EPMC7362003biostudies-literatureMultifunctional and Transformable 'Clickable' Hydrogel Coatings on Titanium Surfaces: From Protein Immobilization to Cellular Attachment.PMC7362003Gevrek TNDegirmenci ASanyal ASanyal RfalseMultifunctional and Transformable 'Clickable' Hydrogel Coatings on Titanium Surfaces: From Protein Immobilization to Cellular Attachment.Multifunctionalizable hydrogel coatings on titanium interfaces are useful in a wide range of biomedical applications utilizing titanium-based materials. In this study, furan-protected maleimide groups containing multi-clickable biocompatible hydrogel layers are fabricated on a titanium surface. Upon thermal treatment, the masked maleimide groups within the hydrogel are converted to thiol-reactive maleimide groups. The thiol-reactive maleimide group allows facile functionalization of these hydrogels through the thiol-maleimide nucleophilic addition and Diels-Alder cycloaddition reactions, under mild conditions. Additionally, the strained alkene unit in the furan-protected maleimide moiety undergoes radical thiol-ene reaction, as well as the inverse-electron-demand Diels-Alder reaction with 2020-01-01T00:00:00Z2020 May2025-04-22T19:28:31.856Z2025-04-06T02:47:44.778ZS-EPMC73620033246652110.3390/polym12061211