{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":45,"searchCount":0},"additional":{"omics_type":["Unknown"],"volume":["9(3)"],"submitter":["Hakim MS"],"pubmed_abstract":["Virus-specific T cell-mediated immunity is severely impaired in chronic hepatitis B virus (HBV) patients. HBV-specific T cells in chronic HBV patients show a low ability to produce cytokines and to exert their cytotoxic activity. A prominent characteristic of these exhausted T cells is overexpression of inhibitory receptor molecules which negatively regulate T cell function. In this study, we examined <i>in vitro</i> regulation of two inhibitory receptor expressions, programmed death 1 (PD-1) and T cell immunoglobulin mucin domain-containing molecule 3 (TIM-3). Peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals were <i>in vitro</i> stimulated with a panel of cytokines. PD-1 and TIM-3 expression levels on CD4<sup>+</sup> and CD8<sup>+</sup> T cells were examined at days 2 and 7 post stimulation. We demonstrated that PD-1 and TIM-3 were induced via polyclonal (anti-CD3) and cytokine (interleukin 15 [IL-15]) stimulations. Noteworthy, there was a significantly increased induction of TIM-3 on CD8<sup>+</sup> T cells as compared to CD4<sup>+</sup> T cells. Our study thus contributes to further understanding the regulation of T cell exhaustion markers PD-1 and TIM-3."],"journal":["American journal of clinical and experimental immunology"],"pagination":["10-21"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7364376"],"repository":["biostudies-literature"],"pubmed_title":["Interleukin 15 upregulates the expression of PD-1 and TIM-3 on CD4<sup>+</sup> and CD8<sup>+</sup> T cells."],"pmcid":["PMC7364376"],"pubmed_authors":["Hakim MS","Jariah ROA","Spaan M","Boonstra A"],"view_count":["45"],"additional_accession":[]},"is_claimable":false,"name":"Interleukin 15 upregulates the expression of PD-1 and TIM-3 on CD4<sup>+</sup> and CD8<sup>+</sup> T cells.","description":"Virus-specific T cell-mediated immunity is severely impaired in chronic hepatitis B virus (HBV) patients. HBV-specific T cells in chronic HBV patients show a low ability to produce cytokines and to exert their cytotoxic activity. A prominent characteristic of these exhausted T cells is overexpression of inhibitory receptor molecules which negatively regulate T cell function. In this study, we examined <i>in vitro</i> regulation of two inhibitory receptor expressions, programmed death 1 (PD-1) and T cell immunoglobulin mucin domain-containing molecule 3 (TIM-3). Peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals were <i>in vitro</i> stimulated with a panel of cytokines. PD-1 and TIM-3 expression levels on CD4<sup>+</sup> and CD8<sup>+</sup> T cells were examined at days 2 and 7 post stimulation. We demonstrated that PD-1 and TIM-3 were induced via polyclonal (anti-CD3) and cytokine (interleukin 15 [IL-15]) stimulations. Noteworthy, there was a significantly increased induction of TIM-3 on CD8<sup>+</sup> T cells as compared to CD4<sup>+</sup> T cells. Our study thus contributes to further understanding the regulation of T cell exhaustion markers PD-1 and TIM-3.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2024-11-21T06:00:09.783Z","creation":"2020-11-19T16:52:37Z"},"accession":"S-EPMC7364376","cross_references":{"pubmed":["32704430"]}}