biostudies-literatureUnknown41Gassler ABundesministerium für Bildung und Forschung<h4>Objective</h4>Altered gene expression contributes to the development of type 2 diabetes (T2D); thus, the analysis of differentially expressed genes between diabetes-susceptible and diabetes-resistant mouse models is an important tool for the determination of candidate genes that participate in the pathology. Based on RNA-seq and array data comparing pancreatic gene expression of diabetes-prone New Zealand Obese (NZO) mice and diabetes-resistant B6.V-ob/ob (B6-ob/ob) mice, the gap junction protein beta 4 (Gjb4) was identified as a putative novel T2D candidate gene.<h4>Methods</h4>Gjb4 was overexpressed in primary islet cells derived from C57BL/6 (B6) mice and INS-1 cells via adenoviral-mediated infection. The proliferation rate of cells was assessed by BrdU incorporation, and insulin seMolecular metabolism101042https://www.ebi.ac.uk/biostudies/studies/S-EPMC7365933biostudies-literatureOverexpression of Gjb4 impairs cell proliferation and insulin secretion in primary islet cells.PMC7365933Jonas WVogel HStadion MMayer FHelms ASpranger JQuiclet CSchwerbel KKluth OWilhelmi IGassler AGottmann POuni MSchurmann AfalseOverexpression of Gjb4 impairs cell proliferation and insulin secretion in primary islet cells.<h4>Objective</h4>Altered gene expression contributes to the development of type 2 diabetes (T2D); thus, the analysis of differentially expressed genes between diabetes-susceptible and diabetes-resistant mouse models is an important tool for the determination of candidate genes that participate in the pathology. Based on RNA-seq and array data comparing pancreatic gene expression of diabetes-prone New Zealand Obese (NZO) mice and diabetes-resistant B6.V-ob/ob (B6-ob/ob) mice, the gap junction protein beta 4 (Gjb4) was identified as a putative novel T2D candidate gene.<h4>Methods</h4>Gjb4 was overexpressed in primary islet cells derived from C57BL/6 (B6) mice and INS-1 cells via adenoviral-mediated infection. The proliferation rate of cells was assessed by BrdU incorporation, and insulin se2020-01-01T00:00:00Z2020 Nov2026-07-16T02:56:41.274Z2025-04-04T13:38:25.255ZS-EPMC73659333256535810.1016/j.molmet.2020.101042