biostudies-literatureKhang MKNational Heart, Lung and Blood InstituteNHLBI NIH HHS1102-1112https://www.ebi.ac.uk/biostudies/studies/S-EPMC7365982biostudies-literatureUnknown5(4)This work details a newly developed "sandwich" nanoplatform via neutravidin-biotin system for the detection and treatment of inflammation. First, biotinylated- and folate-conjugated optical imaging micelles targeted activated macrophages via folate/folate receptor interactions. Second, multivalent neutravidin proteins in an optimal concentration accumulated on the biotinylated macrophages. Finally, biotinylated anti-inflammatory drug-loaded micelles delivered drugs effectively at the inflammatory sites via a highly specific neutravidin-biotin affinity. Both <i>in vitro</i> and <i>in vivo</i> studies have shown that the "sandwich" pretargeting platform was able to diagnose inflammation by targeting activated macrophages as well as improve the therapeutic efficacy by amplifying the drug deliBioactive materialsA pretargeting nanoplatform for imaging and enhancing anti-inflammatory drug delivery.PMC7365982T32 HL134613NIH T32 HL134613Khang MKTang LLi SCo CMZhou JfalseA pretargeting nanoplatform for imaging and enhancing anti-inflammatory drug delivery.This work details a newly developed "sandwich" nanoplatform via neutravidin-biotin system for the detection and treatment of inflammation. First, biotinylated- and folate-conjugated optical imaging micelles targeted activated macrophages via folate/folate receptor interactions. Second, multivalent neutravidin proteins in an optimal concentration accumulated on the biotinylated macrophages. Finally, biotinylated anti-inflammatory drug-loaded micelles delivered drugs effectively at the inflammatory sites via a highly specific neutravidin-biotin affinity. Both <i>in vitro</i> and <i>in vivo</i> studies have shown that the "sandwich" pretargeting platform was able to diagnose inflammation by targeting activated macrophages as well as improve the therapeutic efficacy by amplifying the drug deli2020-01-01T00:00:00Z2020 Dec2025-04-04T13:38:26.814Z2025-04-04T13:38:26.814ZS-EPMC73659823269593910.1016/j.bioactmat.2020.06.019