{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Meng X"],"pubmed_abstract":["Staphylococcal infection is one of the most pressing problems in modern medicine due to the increasing antibiotic resistance with the overuse of antibiotics. Conventional methods for identification and antimicrobial susceptibility testing (AST) generally take 3-7 days and require skilled technicians. In this study, a microfluidic device based on loop-mediated isothermal amplification (LAMP) was developed, which could discriminate <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, <i>Staphylococcus haemolyticus</i>, and <i>Staphylococcus hominis</i> and predict their methicillin resistance by targeting the <i>mecA</i> and <i>femA</i> genes within 70 min including the hands-on time. Multiplex and real-time detection was achieved in a closed system without aerosol contamination."],"journal":["Frontiers in microbiology"],"pagination":["1487"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7367217"],"repository":["biostudies-literature"],"pubmed_title":["Rapid Detection of <i>mecA</i> and <i>femA</i> Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance."],"pmcid":["PMC7367217"],"pubmed_authors":["Zhang G","Kang X","Gao M","Fan Y","Sun B","Shi G","Liu S","Wang Y","Meng X"],"additional_accession":[]},"is_claimable":false,"name":"Rapid Detection of <i>mecA</i> and <i>femA</i> Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance.","description":"Staphylococcal infection is one of the most pressing problems in modern medicine due to the increasing antibiotic resistance with the overuse of antibiotics. Conventional methods for identification and antimicrobial susceptibility testing (AST) generally take 3-7 days and require skilled technicians. In this study, a microfluidic device based on loop-mediated isothermal amplification (LAMP) was developed, which could discriminate <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, <i>Staphylococcus haemolyticus</i>, and <i>Staphylococcus hominis</i> and predict their methicillin resistance by targeting the <i>mecA</i> and <i>femA</i> genes within 70 min including the hands-on time. Multiplex and real-time detection was achieved in a closed system without aerosol contamination.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-05-02T23:47:09.466Z","creation":"2020-11-19T13:36:33Z"},"accession":"S-EPMC7367217","cross_references":{"pubmed":["32754129"],"doi":["10.3389/fmicb.2020.01487"]}}