biostudies-literatureUnknown17(4)Ondrej M<h4>Background</h4>Autophagy is a crucial factor contributing to radioresistance during radiotherapy. Although Lys05 has proven its ability to improve the results of radiotherapy through the inhibition of autophagy, molecular mechanisms of this inhibition remain elusive. We aimed to describe the molecular mechanisms involved in Lys05-induced inhibition of autophagy.<h4>Materials and methods</h4>Radioresistant human non-small cell lung carcinoma cells (H1299, p53-negative) and methods of quantitative phosphoproteomics were employed to define the molecular mechanisms involved in Lys05-induced inhibition of autophagy.<h4>Results</h4>We confirmed that at an early stage after irradiation, autophagy was induced, whereas at a later stage after irradiation, it was inhibited. The early-stage inductCancer genomics & proteomics369-382https://www.ebi.ac.uk/biostudies/studies/S-EPMC7367607biostudies-literatureLys05 - A Promising Autophagy Inhibitor in the Radiosensitization Battle: Phosphoproteomic Perspective.PMC7367607Fabrik IOndrej MTichy AKlimentova JCechakova LfalseLys05 - A Promising Autophagy Inhibitor in the Radiosensitization Battle: Phosphoproteomic Perspective.<h4>Background</h4>Autophagy is a crucial factor contributing to radioresistance during radiotherapy. Although Lys05 has proven its ability to improve the results of radiotherapy through the inhibition of autophagy, molecular mechanisms of this inhibition remain elusive. We aimed to describe the molecular mechanisms involved in Lys05-induced inhibition of autophagy.<h4>Materials and methods</h4>Radioresistant human non-small cell lung carcinoma cells (H1299, p53-negative) and methods of quantitative phosphoproteomics were employed to define the molecular mechanisms involved in Lys05-induced inhibition of autophagy.<h4>Results</h4>We confirmed that at an early stage after irradiation, autophagy was induced, whereas at a later stage after irradiation, it was inhibited. The early-stage induct2020-01-01T00:00:00Z2020 Jul-Aug2025-06-01T03:25:40.515Z2021-02-19T11:41:41ZS-EPMC73676073257658210.21873/cgp.20196