{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Song S"],"funding":["Bundesministerium für Bildung und Forschung"],"pubmed_abstract":["The introduction of alternative CO<sub>2</sub>-fixing pathways in photoautotrophic organism may improve the efficiency of biological carbon fixation such as minimizing the carbon loss due to photorespiration. Here, we analyzed the effects of creating a formate entry point into the primary metabolism of the cyanobacterium <i>Synechocystis</i> sp. PCC 6803. The formate-tetrahydrofolate ligase (FTL) from <i>Methylobacterium extorquens</i> AM1 was expressed in <i>Synechocystis</i> to enable formate assimilation and reducing the loss of fixed carbon in the photorespiratory pathway. Transgenic strains accumulated serine and 3-phosphoglycerate, and consumed more 2-phosphoglycolate and glycine, which seemed to reflect an efficient utilization of formate. However, labeling experiments showed that t"],"journal":["Frontiers in microbiology"],"pagination":["1650"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7372957"],"repository":["biostudies-literature"],"pubmed_title":["Expression of Formate-Tetrahydrofolate Ligase Did Not Improve Growth but Interferes With Nitrogen and Carbon Metabolism of <i>Synechocystis</i> sp. PCC 6803."],"pmcid":["PMC7372957"],"pubmed_authors":["Brouwer EM","Hagemann M","Song S","Timm S","Lindner SN","Reimann V","Hess WR"],"additional_accession":[]},"is_claimable":false,"name":"Expression of Formate-Tetrahydrofolate Ligase Did Not Improve Growth but Interferes With Nitrogen and Carbon Metabolism of <i>Synechocystis</i> sp. PCC 6803.","description":"The introduction of alternative CO<sub>2</sub>-fixing pathways in photoautotrophic organism may improve the efficiency of biological carbon fixation such as minimizing the carbon loss due to photorespiration. Here, we analyzed the effects of creating a formate entry point into the primary metabolism of the cyanobacterium <i>Synechocystis</i> sp. PCC 6803. The formate-tetrahydrofolate ligase (FTL) from <i>Methylobacterium extorquens</i> AM1 was expressed in <i>Synechocystis</i> to enable formate assimilation and reducing the loss of fixed carbon in the photorespiratory pathway. Transgenic strains accumulated serine and 3-phosphoglycerate, and consumed more 2-phosphoglycolate and glycine, which seemed to reflect an efficient utilization of formate. However, labeling experiments showed that t","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-04-20T03:11:05.757Z","creation":"2020-11-19T13:35:04Z"},"accession":"S-EPMC7372957","cross_references":{"pubmed":["32760387"],"doi":["10.3389/fmicb.2020.01650"]}}