biostudies-literatureSeong HJNational Research Foundation (NRF) of KoreaAgricultural Science and Technology Development12132https://www.ebi.ac.uk/biostudies/studies/S-EPMC7376015biostudies-literatureUnknown10(1)Marine biomasses capable of fixing carbon dioxide have attracted attention as an alternative to fossil resources for fuel and chemical production. Although a simple co-fermentation of fermentable sugars, such as glucose and galactose, has been reported from marine biomass, no previous report has discussed the fine-control of the galactose-to-glucose consumption ratio in this context. Here, we sought to finely control the galactose-to-glucose consumption ratio in the co-fermentation of these sugars using engineered Escherichia coli strains. Toward this end, we constructed E. coli strains GR2, GR2P, and GR2PZ by knocking out galRS, galRS-pfkA, and galRS-pfkA-zwf, respectively, in parent strain W3110. We found that strains W3110, GR2, GR2P, and GR2PZ achieved 0.03, 0.09, 0.12, and 0.17 galactScientific reportsControl of the galactose-to-glucose consumption ratio in co-fermentation using engineered Escherichia coli strains.PMC7376015PJ01492601NRF-2019R1A4A1029125Woo JESeong HJJang YSfalseControl of the galactose-to-glucose consumption ratio in co-fermentation using engineered Escherichia coli strains.Marine biomasses capable of fixing carbon dioxide have attracted attention as an alternative to fossil resources for fuel and chemical production. Although a simple co-fermentation of fermentable sugars, such as glucose and galactose, has been reported from marine biomass, no previous report has discussed the fine-control of the galactose-to-glucose consumption ratio in this context. Here, we sought to finely control the galactose-to-glucose consumption ratio in the co-fermentation of these sugars using engineered Escherichia coli strains. Toward this end, we constructed E. coli strains GR2, GR2P, and GR2PZ by knocking out galRS, galRS-pfkA, and galRS-pfkA-zwf, respectively, in parent strain W3110. We found that strains W3110, GR2, GR2P, and GR2PZ achieved 0.03, 0.09, 0.12, and 0.17 galact2020-01-01T00:00:00Z2020 Jul2025-04-05T10:53:20.394Z2025-04-05T10:53:20.394ZS-EPMC73760153269927510.1038/s41598-020-69143-3