{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ayaub EA"],"funding":["Ontario Thoracic Society","Eva Eugenia Lillian Cope Graduate","Ontario Graduate Scholarships","CIHR"],"pagination":["203-217"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7379543"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["97(2)"],"pubmed_abstract":["Although recent evidence has shown that IL-6 is involved in enhanced alternative activation of macrophages toward a profibrotic phenotype, the mechanisms leading to their increased secretory capacity are not fully understood. Here, we investigated the effect of IL-6 on endoplasmic reticulum (ER) expansion and alternative activation of macrophages in vitro. An essential mediator in this ER expansion process is the IRE1 pathway, which possesses a kinase and endoribonuclease domain to cleave XBP1 into a spliced bioactive molecule. To investigate the IRE1-XBP1 expansion pathway, IL-4/IL-13 and IL-4/IL-13/IL-6-mediated alternative programming of murine bone marrow-derived and human THP1 macrophages were assessed by arginase activity in cell lysates, CD206 and arginase-1 expression by flow cytom"],"journal":["Immunology and cell biology"],"pubmed_title":["IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages."],"pmcid":["PMC7379543"],"funding_grant_id":["137013","140358"],"pubmed_authors":["Dickhout JG","Upagupta C","Ayaub EA","Tandon K","Dvorkin-Gheva A","Padwal M","Dubey A","Lhotak S","Mekhael O","Ayoub A","Richards CD","Ask K","Imani J","Kolb MRJ","Kolb PS","Austin RC","Murphy J","Patel H"],"additional_accession":[]},"is_claimable":false,"name":"IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages.","description":"Although recent evidence has shown that IL-6 is involved in enhanced alternative activation of macrophages toward a profibrotic phenotype, the mechanisms leading to their increased secretory capacity are not fully understood. Here, we investigated the effect of IL-6 on endoplasmic reticulum (ER) expansion and alternative activation of macrophages in vitro. An essential mediator in this ER expansion process is the IRE1 pathway, which possesses a kinase and endoribonuclease domain to cleave XBP1 into a spliced bioactive molecule. To investigate the IRE1-XBP1 expansion pathway, IL-4/IL-13 and IL-4/IL-13/IL-6-mediated alternative programming of murine bone marrow-derived and human THP1 macrophages were assessed by arginase activity in cell lysates, CD206 and arginase-1 expression by flow cytom","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Feb","modification":"2025-04-05T13:44:22.441Z","creation":"2025-04-05T13:44:22.441Z"},"accession":"S-EPMC7379543","cross_references":{"pubmed":["30298952"],"doi":["10.1111/imcb.12212"]}}