{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Kim SY"],"funding":["Svenska Forskningsrådet Formas"],"pubmed_abstract":["Callose deposition is induced in plants by various stress factors such as when plants are attacked by herbivores and pathogens. In the case of aphids, callose plugging of aphid-damaged phloem sieve tubes is expected to reduce aphid access to the phloem sap, while aphid-induced upregulation of callose-degrading <i>β</i>-1,3-glucanase genes in the host plant might counteract this negative effect on aphid performance. We have tested this hypothesis with barley mutants in which one or both of two <i>β</i>-1,3-glucanase genes (1636 and 1639) have been mutated by CRISPR/Cas9 technique in cv. Golden Promise. These two genes were previously found to be upregulated by the cereal pest <i>Rhopalosiphum padi</i> L. in susceptible barley genotypes. Four 1636/1639 double mutant, three 1636 single mutant"],"journal":["Frontiers in plant science"],"pagination":["1043"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7381296"],"repository":["biostudies-literature"],"pubmed_title":["Mutations in Two Aphid-Regulated <i>β</i>-1,3-Glucanase Genes by CRISPR/Cas9 Do Not Increase Barley Resistance to <i>Rhopalosiphum padi</i> L."],"pmcid":["PMC7381296"],"pubmed_authors":["Kim SY","Bengtsson T","Zhu LH","Ahman I","Olsson N","Hot V"],"additional_accession":[]},"is_claimable":false,"name":"Mutations in Two Aphid-Regulated <i>β</i>-1,3-Glucanase Genes by CRISPR/Cas9 Do Not Increase Barley Resistance to <i>Rhopalosiphum padi</i> L.","description":"Callose deposition is induced in plants by various stress factors such as when plants are attacked by herbivores and pathogens. In the case of aphids, callose plugging of aphid-damaged phloem sieve tubes is expected to reduce aphid access to the phloem sap, while aphid-induced upregulation of callose-degrading <i>β</i>-1,3-glucanase genes in the host plant might counteract this negative effect on aphid performance. We have tested this hypothesis with barley mutants in which one or both of two <i>β</i>-1,3-glucanase genes (1636 and 1639) have been mutated by CRISPR/Cas9 technique in cv. Golden Promise. These two genes were previously found to be upregulated by the cereal pest <i>Rhopalosiphum padi</i> L. in susceptible barley genotypes. Four 1636/1639 double mutant, three 1636 single mutant","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-05-04T03:43:23.592Z","creation":"2020-11-07T11:38:23Z"},"accession":"S-EPMC7381296","cross_references":{"pubmed":["32754185"],"doi":["10.3389/fpls.2020.01043"]}}