biostudies-literatureUnknown11Kim SYSvenska Forskningsrådet FormasCallose deposition is induced in plants by various stress factors such as when plants are attacked by herbivores and pathogens. In the case of aphids, callose plugging of aphid-damaged phloem sieve tubes is expected to reduce aphid access to the phloem sap, while aphid-induced upregulation of callose-degrading <i>β</i>-1,3-glucanase genes in the host plant might counteract this negative effect on aphid performance. We have tested this hypothesis with barley mutants in which one or both of two <i>β</i>-1,3-glucanase genes (1636 and 1639) have been mutated by CRISPR/Cas9 technique in cv. Golden Promise. These two genes were previously found to be upregulated by the cereal pest <i>Rhopalosiphum padi</i> L. in susceptible barley genotypes. Four 1636/1639 double mutant, three 1636 single mutantFrontiers in plant science1043https://www.ebi.ac.uk/biostudies/studies/S-EPMC7381296biostudies-literatureMutations in Two Aphid-Regulated <i>β</i>-1,3-Glucanase Genes by CRISPR/Cas9 Do Not Increase Barley Resistance to <i>Rhopalosiphum padi</i> L.PMC7381296Kim SYBengtsson TZhu LHAhman IOlsson NHot VfalseMutations in Two Aphid-Regulated <i>β</i>-1,3-Glucanase Genes by CRISPR/Cas9 Do Not Increase Barley Resistance to <i>Rhopalosiphum padi</i> L.Callose deposition is induced in plants by various stress factors such as when plants are attacked by herbivores and pathogens. In the case of aphids, callose plugging of aphid-damaged phloem sieve tubes is expected to reduce aphid access to the phloem sap, while aphid-induced upregulation of callose-degrading <i>β</i>-1,3-glucanase genes in the host plant might counteract this negative effect on aphid performance. We have tested this hypothesis with barley mutants in which one or both of two <i>β</i>-1,3-glucanase genes (1636 and 1639) have been mutated by CRISPR/Cas9 technique in cv. Golden Promise. These two genes were previously found to be upregulated by the cereal pest <i>Rhopalosiphum padi</i> L. in susceptible barley genotypes. Four 1636/1639 double mutant, three 1636 single mutant2020-01-01T00:00:00Z20202026-05-04T03:43:23.592Z2020-11-07T11:38:23ZS-EPMC73812963275418510.3389/fpls.2020.01043