biostudies-literatureUnknown14(2)Saxena AK<h4>Background</h4>The present study has been designed with the aim of evaluating A-kinase anchoring proteins 3 (<i>AKAP3</i>) and Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 3 (<i>PLOD3</i>) gene mutations and prediction of 3D protein structure for ligand binding activity in the cases of non-obstructive azoospermic male.<h4>Materials and methods</h4>Clinically diagnosed cases of non-obstructive azoospermia (n=111) with age matched controls (n=42) were included in the present case-control study for genetics analysis and confirmation of diagnosis. The sample size was calculated using Epi info software version 6 with 90 power and 95% confidence interval. Genomic DNA was isolated from blood (2.0 ml) and a selected case was used for whole exome sequencing (WES) using Illumina Hiseq for idInternational journal of fertility & sterility102-109https://www.ebi.ac.uk/biostudies/studies/S-EPMC7382683biostudies-literaturePrediction of 3D Protein Structure Based on The Mutation of <i>AKAP3</i> and <i>PLOD3</i> Genes in The Case of Non-Obstructive Azoospermia.PMC7382683Agarwal MAniket Kumar ASaxena AKTiwari MfalsePrediction of 3D Protein Structure Based on The Mutation of <i>AKAP3</i> and <i>PLOD3</i> Genes in The Case of Non-Obstructive Azoospermia.<h4>Background</h4>The present study has been designed with the aim of evaluating A-kinase anchoring proteins 3 (<i>AKAP3</i>) and Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 3 (<i>PLOD3</i>) gene mutations and prediction of 3D protein structure for ligand binding activity in the cases of non-obstructive azoospermic male.<h4>Materials and methods</h4>Clinically diagnosed cases of non-obstructive azoospermia (n=111) with age matched controls (n=42) were included in the present case-control study for genetics analysis and confirmation of diagnosis. The sample size was calculated using Epi info software version 6 with 90 power and 95% confidence interval. Genomic DNA was isolated from blood (2.0 ml) and a selected case was used for whole exome sequencing (WES) using Illumina Hiseq for id2020-01-01T00:00:00Z2020 Jul2025-06-01T03:23:13.38Z2020-11-09T08:46:52ZS-EPMC73826833268162110.22074/ijfs.2020.6028