biostudies-literatureNardo ADAustrian Science Fund FWF1620-1633https://www.ebi.ac.uk/biostudies/studies/S-EPMC7384114biostudies-literatureUnknown40(7)<h4>Background & aims</h4>Osteopontin, a multifunctional protein and inflammatory cytokine, is overexpressed in adipose tissue and liver in obesity and contributes to the induction of adipose tissue inflammation and non-alcoholic fatty liver (NAFL). Studies performed in both mice and humans also point to a potential role for OPN in malignant transformation and tumour growth. To fully understand the role of OPN on the development of NAFL-derived hepatocellular carcinoma (HCC), we applied a non-alcoholic steatohepatitis (NASH)-HCC mouse model on osteopontin-deficient (Spp1^-/- ) mice analysing time points of NASH, fibrosis and HCC compared to wild-type mice.<h4>Methods</h4>Two-day-old wild-type and Spp1^-/- mice received a low-dose streptozotocin injection in order to indLiver international : official journal of the International Association for the Study of the LiverImpact of osteopontin on the development of non-alcoholic liver disease and related hepatocellular carcinoma.PMC7384114W1205‐B09Oberhuber GHelbich THMarkgraf DFGrun NGDumanic MRoden MZeyda MNardo ADRivelles ETrauner MStulnig TMClaudel TfalseImpact of osteopontin on the development of non-alcoholic liver disease and related hepatocellular carcinoma.<h4>Background & aims</h4>Osteopontin, a multifunctional protein and inflammatory cytokine, is overexpressed in adipose tissue and liver in obesity and contributes to the induction of adipose tissue inflammation and non-alcoholic fatty liver (NAFL). Studies performed in both mice and humans also point to a potential role for OPN in malignant transformation and tumour growth. To fully understand the role of OPN on the development of NAFL-derived hepatocellular carcinoma (HCC), we applied a non-alcoholic steatohepatitis (NASH)-HCC mouse model on osteopontin-deficient (Spp1^-/- ) mice analysing time points of NASH, fibrosis and HCC compared to wild-type mice.<h4>Methods</h4>Two-day-old wild-type and Spp1^-/- mice received a low-dose streptozotocin injection in order to ind2020-01-01T00:00:00Z2020 Jul2025-04-19T23:02:54.917Z2025-04-19T23:02:54.917ZS-EPMC73841143228124810.1111/liv.14464