{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sheehan H"],"funding":["Swiss National Science Foundation","Biotechnology and Biological Sciences Research Council","National Science Foundation","U.S. Department of Education"],"pagination":["914-929"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7384185"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["227(3)"],"pubmed_abstract":["The evolution of l-DOPA 4,5-dioxygenase activity, encoded by the gene DODA, was a key step in the origin of betalain biosynthesis in Caryophyllales. We previously proposed that l-DOPA 4,5-dioxygenase activity evolved via a single Caryophyllales-specific neofunctionalisation event within the DODA gene lineage. However, this neofunctionalisation event has not been confirmed and the DODA gene lineage exhibits numerous gene duplication events, whose evolutionary significance is unclear. To address this, we functionally characterised 23 distinct DODA proteins for l-DOPA 4,5-dioxygenase activity, from four betalain-pigmented and five anthocyanin-pigmented species, representing key evolutionary transitions across Caryophyllales. By mapping these functional data to an updated DODA phylogeny, we th"],"journal":["The New phytologist"],"pubmed_title":["Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales."],"pmcid":["PMC7384185"],"funding_grant_id":["DEB 1352907","DEB 1354048","GSP DE‐SC0008834","P300PA_174333","165359","174333","P2BEP3_165359"],"pubmed_authors":["Smith SA","Pucker B","Sheehan H","Tiley H","Lopez-Nieves S","Guo R","Sanderson MJ","Moore MJ","Zhao L","Yim WC","Feng T","Copetti D","Timoneda A","Brockington SF","Walker-Hale N","Cushman JC","Badgami R"],"additional_accession":[]},"is_claimable":false,"name":"Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales.","description":"The evolution of l-DOPA 4,5-dioxygenase activity, encoded by the gene DODA, was a key step in the origin of betalain biosynthesis in Caryophyllales. We previously proposed that l-DOPA 4,5-dioxygenase activity evolved via a single Caryophyllales-specific neofunctionalisation event within the DODA gene lineage. However, this neofunctionalisation event has not been confirmed and the DODA gene lineage exhibits numerous gene duplication events, whose evolutionary significance is unclear. To address this, we functionally characterised 23 distinct DODA proteins for l-DOPA 4,5-dioxygenase activity, from four betalain-pigmented and five anthocyanin-pigmented species, representing key evolutionary transitions across Caryophyllales. By mapping these functional data to an updated DODA phylogeny, we th","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Aug","modification":"2025-04-19T23:04:04.448Z","creation":"2025-02-19T02:29:11.346Z"},"accession":"S-EPMC7384185","cross_references":{"pubmed":["31369159"],"doi":["10.1111/nph.16089"]}}