biostudies-literaturePhillips EANational Institute of Allergy and Infectious DiseasesBill & Melinda Gates Foundation - Grand Challenges Explorations InitiativeNIAID NIH HHSPurdue UniversityBill and Melinda Gates FoundationDivision of Graduate Education3375-3386https://www.ebi.ac.uk/biostudies/studies/S-EPMC7384476biostudies-literatureUnknown19(20)While identifying acute HIV infection is critical to providing prompt treatment to HIV-positive individuals and preventing transmission, existing laboratory-based testing methods are too complex to perform at the point of care. Specifically, molecular techniques can detect HIV RNA within 8-10 days of transmission but require laboratory infrastructure for cold-chain reagent storage and extensive sample preparation performed by trained personnel. Here, we demonstrate our point-of-care microfluidic rapid and autonomous analysis device (microRAAD) that automatically detects HIV RNA from whole blood. Inside microRAAD, we incorporate vitrified amplification reagents, thermally-actuated valves for fluidic control, and a temperature control circuit for low-power heating. Reverse transcription loopLab on a chipMicrofluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples.PMC7384476R61AI140474R61 AI140474DGE-1333468OPP1150806Jankowski LALin LKEjendal KFKByers KMHoilett OSBennett JBPhillips EABasing LAStanciu LAMoehling TJLinnes JCfalseMicrofluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples.While identifying acute HIV infection is critical to providing prompt treatment to HIV-positive individuals and preventing transmission, existing laboratory-based testing methods are too complex to perform at the point of care. Specifically, molecular techniques can detect HIV RNA within 8-10 days of transmission but require laboratory infrastructure for cold-chain reagent storage and extensive sample preparation performed by trained personnel. Here, we demonstrate our point-of-care microfluidic rapid and autonomous analysis device (microRAAD) that automatically detects HIV RNA from whole blood. Inside microRAAD, we incorporate vitrified amplification reagents, thermally-actuated valves for fluidic control, and a temperature control circuit for low-power heating. Reverse transcription loop2019-01-01T00:00:00Z2019 Oct2025-04-19T23:05:12.278Z2025-04-19T23:05:12.278ZS-EPMC73844763153900110.1039/c9lc00506d