biostudies-literatureWan MJiangsu Natural Science FoundationJiangsu Provincial Key Medical Talent Foundation, and Six Talent Peaks Project of Jiangsu ProvinceNational Science Foundation of ChinaNational Natural Science Foundation of ChinaJiangsu Key Technology RD ProgramExcellent Young Scholars NSFCSocial Development Project of Jiangsu Provincial Science and Technology DepartmentTechnology Support Program of Science and Technology Department of Jiangsu ProvinceHuaian Biofunctional Materials and Analysis Technology Innovation Platformeaaz9014https://www.ebi.ac.uk/biostudies/studies/S-EPMC7385437biostudies-literatureUnknown6(22)The treatment difficulties of venous thrombosis include short half-life, low utilization, and poor penetration of drugs at thrombus site. Here, we develop one kind of mesoporous/macroporous silica/platinum nanomotors with platelet membrane (PM) modification (MMNM/PM) for sequentially targeting delivery of thrombolytic and anticoagulant drugs for thrombus treatment. Regulated by the special proteins on PM, the nanomotors target the thrombus site and then PM can be ruptured under near-infrared (NIR) irradiation to achieve desirable sequential drug release, including rapid release of thrombolytic urokinase (3 hours) and slow release of anticoagulant heparin (>20 days). Meantime, the motion ability of nanomotors under NIR irradiation can effectively promote them to penetrate deeply in thrombus site to enhance retention ratio. The in vitro and in vivo evaluation results confirm that the synergistic effect of targeting ability from PM and motion ability from nanomotors can notably enhance the thrombolysis effect in both static/dynamic thrombus and rat model.Science advancesPlatelet-derived porous nanomotor for thrombus therapy.PMC7385437HAP2016128162203381572129BE2015703WSW-061BE201660921603105, 21571104BE2019744),BE2016010, BE2015603Fang DZhao BWan MJiang QWang FWang QHuang YMao CWang RLi TZhang YWu RShi DWang XXu XFang LMiao ZYu YfalsePlatelet-derived porous nanomotor for thrombus therapy.The treatment difficulties of venous thrombosis include short half-life, low utilization, and poor penetration of drugs at thrombus site. Here, we develop one kind of mesoporous/macroporous silica/platinum nanomotors with platelet membrane (PM) modification (MMNM/PM) for sequentially targeting delivery of thrombolytic and anticoagulant drugs for thrombus treatment. Regulated by the special proteins on PM, the nanomotors target the thrombus site and then PM can be ruptured under near-infrared (NIR) irradiation to achieve desirable sequential drug release, including rapid release of thrombolytic urokinase (3 hours) and slow release of anticoagulant heparin (>20 days). Meantime, the motion ability of nanomotors under NIR irradiation can effectively promote them to penetrate deeply in thrombus site to enhance retention ratio. The in vitro and in vivo evaluation results confirm that the synergistic effect of targeting ability from PM and motion ability from nanomotors can notably enhance the thrombolysis effect in both static/dynamic thrombus and rat model.2020-01-01T00:00:00Z2020 May2025-04-04T07:40:49.447Z2025-04-04T07:40:49.447ZS-EPMC73854373276644510.1126/sciadv.aaz9014