biostudies-literatureDi Paola MAutonomous Province of TrentoUniversity of FlorenceIntegrative Project SYBARISSeventh Framework ProgrammeMinistero dell’ Istruzione, dell’ Università e della Ricerca100036https://www.ebi.ac.uk/biostudies/studies/S-EPMC7388382biostudies-literatureUnknown3Investigation of the fungal communities in animal models of Inflammatory Bowel Diseases (IBD) showed a controversial role of <i>Saccharomyces cerevisiae</i> and <i>Candida</i> spp<i>.</i> In health and disease. These conflicting observations could be ascribed to immunogenic differences among co-specific strains. To assess the relevance of intra-strains differences on yeast immunogenicity and impact on the microbiota, we screened <i>S. cerevisiae</i> and <i>Candida</i> spp. Strains isolated from fecal samples of IBD patients. We compared the cytokine profiles, obtained upon stimulation of Peripheral Blood Mononuclear Cells (PBMCs) and Dendritic Cells with different yeast strains, and evaluated the relationship between strain's cell wall sugar amount and immune response. Moreover, the gut miJournal of translational autoimmunityComparative immunophenotyping of <i>Saccharomyces cerevisiae</i> and <i>Candida</i> spp. strains from Crohn's disease patients and their interactions with the gut microbiome.PMC7388382FP7/2007–2013HEALTH-2010-242,220PRIN 2007-N352CP_001Lionetti PDe Filippo CCavalieri DDi Paola MRomani LRamazzotti MTocci NRizzetto LVitali FStefanini IMassi-Benedetti CfalseComparative immunophenotyping of <i>Saccharomyces cerevisiae</i> and <i>Candida</i> spp. strains from Crohn's disease patients and their interactions with the gut microbiome.Investigation of the fungal communities in animal models of Inflammatory Bowel Diseases (IBD) showed a controversial role of <i>Saccharomyces cerevisiae</i> and <i>Candida</i> spp<i>.</i> In health and disease. These conflicting observations could be ascribed to immunogenic differences among co-specific strains. To assess the relevance of intra-strains differences on yeast immunogenicity and impact on the microbiota, we screened <i>S. cerevisiae</i> and <i>Candida</i> spp. Strains isolated from fecal samples of IBD patients. We compared the cytokine profiles, obtained upon stimulation of Peripheral Blood Mononuclear Cells (PBMCs) and Dendritic Cells with different yeast strains, and evaluated the relationship between strain's cell wall sugar amount and immune response. Moreover, the gut mi2020-01-01T00:00:00Z20202026-05-04T23:41:59.268Z2020-11-19T13:36:41ZS-EPMC73883823274352010.1016/j.jtauto.2020.100036