biostudies-literatureChu YClinical Technology Development Foundation of Jiangsu UniversityJiangsu Government Scholarship for Overseas StudiesFoundation of the Science and Technology Project of WujinFoundation of the Youth Science and Technology Project of Changzhou Health and Family Planning CommissionChangzhou High-Level Medical Talents Training ProjectSpecial Fund for Clinical Medicine of Jiangsu UniversityInnovative and Entrepreneurial Plan of Jiangsu Province227https://www.ebi.ac.uk/biostudies/studies/S-EPMC7388529biostudies-literatureUnknown20(1)<h4>Background</h4>Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated.<h4>Results</h4>Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB aBMC microbiologyWogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways.PMC7388529WS201402WS201710QN201505JDLC2X020JLY201600382016CZBJ060Xu LYun ZWu ZSu ALv XFu XChu YSong SZhang LWang YChen DfalseWogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways.<h4>Background</h4>Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated.<h4>Results</h4>Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB a2020-01-01T00:00:00Z2020 Jul2025-04-05T15:21:14.57Z2024-11-15T16:29:38.669ZS-EPMC73885293272330010.1186/s12866-020-01916-2