{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Saragliadis A"],"funding":["Novo Nordisk Fonden"],"pagination":["100025"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7388967"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5"],"pubmed_abstract":["We set out to develop scalable assays to measure bacterial adhesion to mammalian extracellular matrix proteins, with the aim to perform high-throughput screening for inhibitors. Our model system is the trimeric autotransporter adhesin YadA from <i>Yersinia enterocolitica</i> that binds to collagen. Using bacterial cells expressing GFP under an inducible promotor, and co-expressing the adhesin of choice, we were able to establish a 384-well plate-based assay that allowed us to screen 28,000 compounds in 8 days (3520 compounds per day). We have collected all parameters that were essential in assay development, and describe how they can be tuned for improved performance. Out of 28,000 compounds, 5 compounds showed significant inhibitory activity, measured as loss of fluorescence compared to c"],"journal":["Cell surface (Amsterdam, Netherlands)"],"pubmed_title":["Assay development for the discovery of small-molecule inhibitors of YadA adhesion to collagen."],"pmcid":["PMC7388967"],"funding_grant_id":["NNF18OC0032818"],"pubmed_authors":["Saragliadis A","Linke D"],"additional_accession":[]},"is_claimable":false,"name":"Assay development for the discovery of small-molecule inhibitors of YadA adhesion to collagen.","description":"We set out to develop scalable assays to measure bacterial adhesion to mammalian extracellular matrix proteins, with the aim to perform high-throughput screening for inhibitors. Our model system is the trimeric autotransporter adhesin YadA from <i>Yersinia enterocolitica</i> that binds to collagen. Using bacterial cells expressing GFP under an inducible promotor, and co-expressing the adhesin of choice, we were able to establish a 384-well plate-based assay that allowed us to screen 28,000 compounds in 8 days (3520 compounds per day). We have collected all parameters that were essential in assay development, and describe how they can be tuned for improved performance. Out of 28,000 compounds, 5 compounds showed significant inhibitory activity, measured as loss of fluorescence compared to c","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2025-04-05T15:21:00.584Z","creation":"2025-04-05T15:21:00.584Z"},"accession":"S-EPMC7388967","cross_references":{"pubmed":["32743141"],"doi":["10.1016/j.tcsw.2019.100025"]}}