biostudies-literatureMurcia ONCI NIH HHS2820-2827https://www.ebi.ac.uk/biostudies/studies/S-EPMC7396148biostudies-literatureUnknown24(12)Purpose: A recent study reported that 5-fluorouracil (5-FU)-based chemotherapy is less effective in treating patients with advanced colorectal cancer demonstrating hypermethylation of the TFAP2E gene. The aim of our study was to confirm and validate these findings in large, uniformly treated, well-characterized patient cohorts.Experimental Design: Two cohorts of 783 patients with colorectal cancer: 532 from a population-based, multicenter cohort (EPICOLON I) and 251 patients from a clinic-based trial were used to study the effectiveness of TFAP2E methylation and expression as a predictor of response of colorectal cancer patients to 5-FU-based chemotherapy. DNA methylation status of the TFAP2E gene in patients with colorectal cancer was assessed by quantitative bisulfite pyrosequencing analClinical cancer research : an official journal of the American Association for Cancer ResearchTFAP2E Methylation and Expression Status Does Not Predict Response to 5-FU-based Chemotherapy in Colorectal Cancer.PMC7396148R01 CA181572R01 CA072851U01 CA187956R01 CA202797R01 CA184792Murcia OPerez-Carbonell LHernandez-Illan EGoel ARojas EBalaguer FAndreu MLlor XCastells AJuarez MJover RAlenda CEgoavil CBoland CRfalseTFAP2E Methylation and Expression Status Does Not Predict Response to 5-FU-based Chemotherapy in Colorectal Cancer.Purpose: A recent study reported that 5-fluorouracil (5-FU)-based chemotherapy is less effective in treating patients with advanced colorectal cancer demonstrating hypermethylation of the TFAP2E gene. The aim of our study was to confirm and validate these findings in large, uniformly treated, well-characterized patient cohorts.Experimental Design: Two cohorts of 783 patients with colorectal cancer: 532 from a population-based, multicenter cohort (EPICOLON I) and 251 patients from a clinic-based trial were used to study the effectiveness of TFAP2E methylation and expression as a predictor of response of colorectal cancer patients to 5-FU-based chemotherapy. DNA methylation status of the TFAP2E gene in patients with colorectal cancer was assessed by quantitative bisulfite pyrosequencing anal2018-01-01T00:00:00Z2018 Jun2025-06-01T02:34:34.272Z2020-10-29T10:28:21ZS-EPMC73961482953512710.1158/1078-0432.CCR-17-294010.1158/1078-0432.ccr-17-2940