biostudies-literatureWoods EJNational Institute of Allergy and Infectious Diseases300https://www.ebi.ac.uk/biostudies/studies/S-EPMC7405448biostudies-literatureUnknown18(1)<h4>Background</h4>Deceased organ donors represent an untapped source of therapeutic bone marrow (BM) that can be recovered in 3-5 times the volume of that obtained from living donors, tested for quality, cryopreserved, and banked indefinitely for future on-demand use. A challenge for a future BM banking system will be to manage the prolonged ischemia times that are inevitable when bones procured at geographically-dispersed locations are shipped to distant facilities for processing. Our objectives were to: (a) quantify, under realistic field conditions, the relationship between ischemia time and the quality of hematopoietic stem and progenitor cells (HSPCs) derived from deceased-donor BM; (b) identify ischemia-time boundaries beyond which HSPC quality is adversely affected; (c) investigateJournal of translational medicineIschemia considerations for the development of an organ and tissue donor derived bone marrow bank.PMC74054481R43AI129444-01A1Sherry AMWoods EJBrandacher GWoods JRHardin JWLaFontaine MJohnstone BHfalseIschemia considerations for the development of an organ and tissue donor derived bone marrow bank.<h4>Background</h4>Deceased organ donors represent an untapped source of therapeutic bone marrow (BM) that can be recovered in 3-5 times the volume of that obtained from living donors, tested for quality, cryopreserved, and banked indefinitely for future on-demand use. A challenge for a future BM banking system will be to manage the prolonged ischemia times that are inevitable when bones procured at geographically-dispersed locations are shipped to distant facilities for processing. Our objectives were to: (a) quantify, under realistic field conditions, the relationship between ischemia time and the quality of hematopoietic stem and progenitor cells (HSPCs) derived from deceased-donor BM; (b) identify ischemia-time boundaries beyond which HSPC quality is adversely affected; (c) investigate2020-01-01T00:00:00Z2020 Aug2026-05-03T20:38:53.662Z2020-08-15T07:03:25ZS-EPMC74054483275826110.1186/s12967-020-02470-1