{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang K"],"funding":["Fundamental Research Funds for the Central Universities","National Basic Research Program of China"],"pagination":["1658"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7411085"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["In our previous study, a two-component-system (TCS) RspA1/A2 was identified and proven to play a positive role in the regulation of salinomycin (antibiotic) biosynthesis in <i>Streptomyces albus</i>. However, the regulatory mechanism of RspA1/A2 using a carbon source (glucose or acetate) for the cell growth of <i>S. albus</i> is still unclear till present research work. Therefore, in this work, the mechanistic pathway of RspA1/A2 on carbon source metabolism is unveiled. Firstly, this work reports that the response regulator RspA1 gene <i>rspA1</i> knocked-out mutant ΔrspA1 exhibits lower biomass accumulation and lower glucose consumption rates as compared to the parental strain A30 when cultivated in a defined minimal medium (MM) complemented with 75 mM glutamate. Further, it is demonstrat"],"journal":["Frontiers in microbiology"],"pubmed_title":["Two-Component-System RspA1/A2-Dependent Regulation on Primary Metabolism in <i>Streptomyces albus</i> A30 Cultivated With Glutamate as the Sole Nitrogen Source."],"pmcid":["PMC7411085"],"funding_grant_id":["No.22221817014","No.2019YFA0904800","No.22221818014"],"pubmed_authors":["Guo M","Zhang K","Hu Y","Chen Z","Ali MF","Chu J","Yu J","Mohsin A","Zhuang Y"],"additional_accession":[]},"is_claimable":false,"name":"Two-Component-System RspA1/A2-Dependent Regulation on Primary Metabolism in <i>Streptomyces albus</i> A30 Cultivated With Glutamate as the Sole Nitrogen Source.","description":"In our previous study, a two-component-system (TCS) RspA1/A2 was identified and proven to play a positive role in the regulation of salinomycin (antibiotic) biosynthesis in <i>Streptomyces albus</i>. However, the regulatory mechanism of RspA1/A2 using a carbon source (glucose or acetate) for the cell growth of <i>S. albus</i> is still unclear till present research work. Therefore, in this work, the mechanistic pathway of RspA1/A2 on carbon source metabolism is unveiled. Firstly, this work reports that the response regulator RspA1 gene <i>rspA1</i> knocked-out mutant ΔrspA1 exhibits lower biomass accumulation and lower glucose consumption rates as compared to the parental strain A30 when cultivated in a defined minimal medium (MM) complemented with 75 mM glutamate. Further, it is demonstrat","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-05-02T06:02:53.578Z","creation":"2020-08-29T07:21:31Z"},"accession":"S-EPMC7411085","cross_references":{"pubmed":["32849342"],"doi":["10.3389/fmicb.2020.01658"]}}