biostudies-literatureZhang KFundamental Research Funds for the Central UniversitiesNational Basic Research Program of China1658https://www.ebi.ac.uk/biostudies/studies/S-EPMC7411085biostudies-literatureUnknown11In our previous study, a two-component-system (TCS) RspA1/A2 was identified and proven to play a positive role in the regulation of salinomycin (antibiotic) biosynthesis in <i>Streptomyces albus</i>. However, the regulatory mechanism of RspA1/A2 using a carbon source (glucose or acetate) for the cell growth of <i>S. albus</i> is still unclear till present research work. Therefore, in this work, the mechanistic pathway of RspA1/A2 on carbon source metabolism is unveiled. Firstly, this work reports that the response regulator RspA1 gene <i>rspA1</i> knocked-out mutant ΔrspA1 exhibits lower biomass accumulation and lower glucose consumption rates as compared to the parental strain A30 when cultivated in a defined minimal medium (MM) complemented with 75 mM glutamate. Further, it is demonstratFrontiers in microbiologyTwo-Component-System RspA1/A2-Dependent Regulation on Primary Metabolism in <i>Streptomyces albus</i> A30 Cultivated With Glutamate as the Sole Nitrogen Source.PMC7411085No.22221817014No.2019YFA0904800No.22221818014Guo MZhang KHu YChen ZAli MFChu JYu JMohsin AZhuang YfalseTwo-Component-System RspA1/A2-Dependent Regulation on Primary Metabolism in <i>Streptomyces albus</i> A30 Cultivated With Glutamate as the Sole Nitrogen Source.In our previous study, a two-component-system (TCS) RspA1/A2 was identified and proven to play a positive role in the regulation of salinomycin (antibiotic) biosynthesis in <i>Streptomyces albus</i>. However, the regulatory mechanism of RspA1/A2 using a carbon source (glucose or acetate) for the cell growth of <i>S. albus</i> is still unclear till present research work. Therefore, in this work, the mechanistic pathway of RspA1/A2 on carbon source metabolism is unveiled. Firstly, this work reports that the response regulator RspA1 gene <i>rspA1</i> knocked-out mutant ΔrspA1 exhibits lower biomass accumulation and lower glucose consumption rates as compared to the parental strain A30 when cultivated in a defined minimal medium (MM) complemented with 75 mM glutamate. Further, it is demonstrat2020-01-01T00:00:00Z20202026-05-02T06:02:53.578Z2020-08-29T07:21:31ZS-EPMC74110853284934210.3389/fmicb.2020.01658