biostudies-literatureSong YNational Natural Science Foundation of China1880https://www.ebi.ac.uk/biostudies/studies/S-EPMC7412599biostudies-literatureUnknown11<i>Exophiala spinifera</i>, a capsule-producing black yeast, is overrepresented as agent of disseminated infection in humans with inherited dysfunction of the <i>CARD9</i> gene. In a review of published caspase recruitment domain-containing protein 9 (CARD9) deficiency cases, black fungi were linked to mutations other than those prevalent in yeast and dermatophyte cases, and were found to respond to a larger panel of cytokines. Here, we sequenced and annotated the genomes of BMU 08022 from a patient with CARD9 deficiency and two environmental strains, BMU 00051 and BMU 00047. We performed genomic and transcriptomic analysis for these isolates including published black yeasts genomes, using a combination of long-read (PACBIO) and short-read (Illumina) sequencing technologies with a hybrid aFrontiers in microbiologyComparative Analysis of Clinical and Environmental Strains of <i>Exophiala spinifera</i> by Long-Reads Sequencing and RNAseq Reveal Adaptive Strategies.PMC74125993167014581520108026Menezes da Silva NSybren de Hoog GLi RVicente VADu MSong YYang EfalseComparative Analysis of Clinical and Environmental Strains of <i>Exophiala spinifera</i> by Long-Reads Sequencing and RNAseq Reveal Adaptive Strategies.<i>Exophiala spinifera</i>, a capsule-producing black yeast, is overrepresented as agent of disseminated infection in humans with inherited dysfunction of the <i>CARD9</i> gene. In a review of published caspase recruitment domain-containing protein 9 (CARD9) deficiency cases, black fungi were linked to mutations other than those prevalent in yeast and dermatophyte cases, and were found to respond to a larger panel of cytokines. Here, we sequenced and annotated the genomes of BMU 08022 from a patient with CARD9 deficiency and two environmental strains, BMU 00051 and BMU 00047. We performed genomic and transcriptomic analysis for these isolates including published black yeasts genomes, using a combination of long-read (PACBIO) and short-read (Illumina) sequencing technologies with a hybrid a2020-01-01T00:00:00Z20202026-04-08T00:37:10.575Z2020-08-29T07:22:09ZS-EPMC74125993284946210.3389/fmicb.2020.01880