biostudies-literatureShan NLNational Institute of Environmental Health SciencesNCCIH NIH HHSNIEHS NIH HHSNCI NIH HHSNational Center for Complementary and Integrative Health of the NIH673-686https://www.ebi.ac.uk/biostudies/studies/S-EPMC7415686biostudies-literatureUnknown13(8)Ductal carcinoma <i>in situ</i> (DCIS), which accounts for one out of every five new breast cancer diagnoses, will progress to potentially lethal invasive ductal carcinoma (IDC) in about 50% of cases. Vitamin D compounds have been shown to inhibit progression to IDC in the MCF10DCIS model. This inhibition appears to involve a reduction in the cancer stem cell-like population in MCF10DCIS tumors. To identify genes that are involved in the vitamin D effects, a global transcriptomic analysis was undertaken of MCF10DCIS cells grown in mammosphere cultures, in which cancer stem-like cells grow preferentially and produce colonies by self-renewal and maturation, in the presence and absence of 1α25(OH)₂D₃ and a vitamin D analog, BXL0124. Using next-generation RNA-sequencing, Cancer prevention research (Philadelphia, Pa.)Analysis of the Transcriptome: Regulation of Cancer Stemness in Breast Ductal Carcinoma <i>In Situ</i> by Vitamin D Compounds.PMC7415686R01 AT009152ES005022R01 CA127645P30 ES005022R01 AT007036Furmanski PCheng DLi SNSargsyan DMaehr HWernyj RKuo HDCai LKong ANMinden ABak MJWu RShan NLFang MSuh NfalseAnalysis of the Transcriptome: Regulation of Cancer Stemness in Breast Ductal Carcinoma <i>In Situ</i> by Vitamin D Compounds.Ductal carcinoma <i>in situ</i> (DCIS), which accounts for one out of every five new breast cancer diagnoses, will progress to potentially lethal invasive ductal carcinoma (IDC) in about 50% of cases. Vitamin D compounds have been shown to inhibit progression to IDC in the MCF10DCIS model. This inhibition appears to involve a reduction in the cancer stem cell-like population in MCF10DCIS tumors. To identify genes that are involved in the vitamin D effects, a global transcriptomic analysis was undertaken of MCF10DCIS cells grown in mammosphere cultures, in which cancer stem-like cells grow preferentially and produce colonies by self-renewal and maturation, in the presence and absence of 1α25(OH)₂D₃ and a vitamin D analog, BXL0124. Using next-generation RNA-sequencing, 2020-01-01T00:00:00Z2020 Aug2026-05-03T04:59:02.586Z2021-02-21T05:14:11ZS-EPMC74156863246729110.1158/1940-6207.capr-19-056610.1158/1940-6207.CAPR-19-0566