{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Anand R"],"funding":["Cardio Pulmonary Institute","DFG","Deutsche Forschungsgemeinschaft","DFG, German Research Foundation","Heinrich Heine University Düsseldorf","Bundesministerium für Bildung und Forschung (BMBF) mitoNET"],"pagination":["e202000711"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7425215"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(10)"],"pubmed_abstract":["Homologous apolipoproteins of MICOS complex, MIC26 and MIC27, show an antagonistic regulation of their protein levels, making it difficult to deduce their individual functions using a single gene deletion. We obtained single and double knockout (DKO) human cells of <i>MIC26</i> and <i>MIC27</i> and found that DKO show more concentric onion-like cristae with loss of CJs than any single deletion indicating overlapping roles in formation of CJs. Using a combination of complexome profiling, STED nanoscopy, and blue-native gel electrophoresis, we found that MIC26 and MIC27 are dispensable for the stability and integration of the remaining MICOS subunits into the complex suggesting that they assemble late into the MICOS complex. MIC26 and MIC27 are cooperatively required for the integrity of res"],"journal":["Life science alliance"],"pubmed_title":["MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes."],"pmcid":["PMC7425215"],"funding_grant_id":["815/Z1","EXC2026","Foko-02/2015","01GM1906D","FoKo-37/2015","RE 1575/2-1","1208"],"pubmed_authors":["Brocke-Ahmadinejad N","Stork B","Golombek M","Wittig I","Kondadi AK","Anand R","Schlutermann D","Peifer-Weiß L","Meisterknecht J","Nortmann O","Reichert AS","Eichmann TO","Riedel J"],"additional_accession":[]},"is_claimable":false,"name":"MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.","description":"Homologous apolipoproteins of MICOS complex, MIC26 and MIC27, show an antagonistic regulation of their protein levels, making it difficult to deduce their individual functions using a single gene deletion. We obtained single and double knockout (DKO) human cells of <i>MIC26</i> and <i>MIC27</i> and found that DKO show more concentric onion-like cristae with loss of CJs than any single deletion indicating overlapping roles in formation of CJs. Using a combination of complexome profiling, STED nanoscopy, and blue-native gel electrophoresis, we found that MIC26 and MIC27 are dispensable for the stability and integration of the remaining MICOS subunits into the complex suggesting that they assemble late into the MICOS complex. MIC26 and MIC27 are cooperatively required for the integrity of res","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2026-05-04T04:27:38.872Z","creation":"2020-08-30T07:19:42Z"},"accession":"S-EPMC7425215","cross_references":{"pubmed":["32788226"],"doi":["10.26508/lsa.202000711"]}}