biostudies-literatureUnknown9(11)Prausmuller SBackground Neprilysin is a transmembrane endopeptidase involved in the breakdown of a variety of vasoactive peptides and serves as a therapeutic target in heart failure with reduced ejection fraction (HFrEF). This study aimed to investigate the relationship of circulating neprilysin with neurohumoral activation and the impact of plasma neprilysin activity on prognosis in HFrEF. Methods and Results A total of 369 chronic HFrEF patients were enrolled prospectively. Plasma neprilysin concentration and activity were determined by a specific ELISA and a fluorometric method. The association between plasma neprilysin and heart failure (HF) severity, neurohumoral activation, ie norepinephrine and absolute renin concentration, as well as all-cause mortality was assessed. Median plasma neprilysin coJournal of the American Heart Associatione015071https://www.ebi.ac.uk/biostudies/studies/S-EPMC7428996biostudies-literaturePlasma Neprilysin Displays No Relevant Association With Neurohumoral Activation in Chronic HFrEF.PMC7428996Hulsmann MFreitag CGoliasch GBartko PEArfsten HSpinka GStrunk GPavo NPrausmuller SfalsePlasma Neprilysin Displays No Relevant Association With Neurohumoral Activation in Chronic HFrEF.Background Neprilysin is a transmembrane endopeptidase involved in the breakdown of a variety of vasoactive peptides and serves as a therapeutic target in heart failure with reduced ejection fraction (HFrEF). This study aimed to investigate the relationship of circulating neprilysin with neurohumoral activation and the impact of plasma neprilysin activity on prognosis in HFrEF. Methods and Results A total of 369 chronic HFrEF patients were enrolled prospectively. Plasma neprilysin concentration and activity were determined by a specific ELISA and a fluorometric method. The association between plasma neprilysin and heart failure (HF) severity, neurohumoral activation, ie norepinephrine and absolute renin concentration, as well as all-cause mortality was assessed. Median plasma neprilysin co2020-01-01T00:00:00Z2020 Jun2025-04-27T00:00:32.555Z2020-10-04T07:17:21ZS-EPMC74289963242703410.1161/JAHA.119.015071