biostudies-literatureChen CNational Key Research and Development Program of China Stem Cell and Translational Research1861https://www.ebi.ac.uk/biostudies/studies/S-EPMC7434936biostudies-literatureUnknown11Organic greenhouse farming is an innovative system that may maintain a high yield and healthy agroecosystem. There have been no rigorous studies on the comparison of total and nitrogen-cycling bacterial community in vegetable soils between organic and conventional farming management at large scale. A survey of bacterial community and nitrogen cycles from soils under organic and conventional greenhouse farming was performed at 30 sites, covering seven soil types with 4 to 18 years of organic farming history. Communities of the total, diazotrophs and ammonia-oxidizing bacteria were studied with high-throughput sequencing of the 16S <i>rRNA</i>, <i>nifH</i> and <i>amoA</i> genes, respectively. Organic greenhouse farming did not influence alpha diversities. Beta diversities among the total (26Frontiers in microbiologyComparison of the Total, Diazotrophic and Ammonia-Oxidizing Bacterial Communities Between Under Organic and Conventional Greenhouse Farming.PMC74349362018YFD05002022016YFD05014042016YFD0800602Qiao YLi JLv YXu THan HHu KChen CLi XXDing GCfalseComparison of the Total, Diazotrophic and Ammonia-Oxidizing Bacterial Communities Between Under Organic and Conventional Greenhouse Farming.Organic greenhouse farming is an innovative system that may maintain a high yield and healthy agroecosystem. There have been no rigorous studies on the comparison of total and nitrogen-cycling bacterial community in vegetable soils between organic and conventional farming management at large scale. A survey of bacterial community and nitrogen cycles from soils under organic and conventional greenhouse farming was performed at 30 sites, covering seven soil types with 4 to 18 years of organic farming history. Communities of the total, diazotrophs and ammonia-oxidizing bacteria were studied with high-throughput sequencing of the 16S <i>rRNA</i>, <i>nifH</i> and <i>amoA</i> genes, respectively. Organic greenhouse farming did not influence alpha diversities. Beta diversities among the total (262020-01-01T00:00:00Z20202026-05-05T00:39:11.543Z2020-09-06T07:06:01ZS-EPMC74349363290333810.3389/fmicb.2020.01861